Comparative ability of IL-12 and IL-28B to regulate Treg populations and enhance adaptive cellular immunity Export

@article{citeulike:4769771, abstract = {Improving the potency of immune responses is paramount among issues concerning vaccines against deadly pathogens. IL-28B belongs to the newly described interferon lambda (IFN{lambda}) family of cytokines, and has not yet been assessed for its potential ability to influence adaptive immune responses or act as a vaccine adjuvant. We compared the ability of plasmid-encoded IL-28B to boost immune responses to a multiclade consensus HIV Gag plasmid during DNA vaccination with that of IL-12. We show here that IL-28B, like IL-12, is capable of robustly enhancing adaptive immunity. Moreover, we describe for the first time how IL-28B reduces regulatory T-cell populations during DNA vaccination, whereas IL-12 increases this cellular subset. We also show that IL-28B, unlike IL-12, is able to increase the percentage of splenic CD8+ T cells in vaccinated animals, and that these cells are more granular and have higher antigen-specific cytolytic degranulation compared with cells taken from animals that received IL-12 as an adjuvant. Lastly, we report that IL-28B can induce 100\% protection from mortality after a lethal influenza challenge. These data suggest that IL-28B is a strong candidate for further studies of vaccine or immunotherapy protocols. 10.1182/blood-2008-11-190520}, author = {Morrow, Matthew P. and Pankhong, Panyupa and Laddy, Dominick J. and Schoenly, Kimberly A. and Yan, Jian and Cisper, Neil and Weiner, David B.}, citeulike-article-id = {4769771}, citeulike-linkout-0 = {http://dx.doi.org/10.1182/blood-2008-11-190520}, citeulike-linkout-1 = {http://bloodjournal.hematologylibrary.org/cgi/content/abstract/113/23/5868?etoc}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19304955}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19304955}, day = {4}, doi = {10.1182/blood-2008-11-190520}, journal = {Blood}, keywords = {adaptive\_immunity, ifns\_type\_iii, il-12, il-28\_inf-lambda3, immune\_regulation, t\_cells, treg\_cells}, month = {June}, number = {23}, pages = {5868--5877}, posted-at = {2009-06-07 20:14:08}, priority = {2}, title = {Comparative ability of IL-12 and IL-28B to regulate Treg populations and enhance adaptive cellular immunity}, url = {http://dx.doi.org/10.1182/blood-2008-11-190520}, volume = {113}, year = {2009} }

TY - JOUR ID - citeulike:4769771 L3 - citeulike-article-id:4769771 N2 - Improving the potency of immune responses is paramount among issues concerning vaccines against deadly pathogens. IL-28B belongs to the newly described interferon lambda (IFN{lambda}) family of cytokines, and has not yet been assessed for its potential ability to influence adaptive immune responses or act as a vaccine adjuvant. We compared the ability of plasmid-encoded IL-28B to boost immune responses to a multiclade consensus HIV Gag plasmid during DNA vaccination with that of IL-12. We show here that IL-28B, like IL-12, is capable of robustly enhancing adaptive immunity. Moreover, we describe for the first time how IL-28B reduces regulatory T-cell populations during DNA vaccination, whereas IL-12 increases this cellular subset. We also show that IL-28B, unlike IL-12, is able to increase the percentage of splenic CD8+ T cells in vaccinated animals, and that these cells are more granular and have higher antigen-specific cytolytic degranulation compared with cells taken from animals that received IL-12 as an adjuvant. Lastly, we report that IL-28B can induce 100% protection from mortality after a lethal influenza challenge. These data suggest that IL-28B is a strong candidate for further studies of vaccine or immunotherapy protocols. 10.1182/blood-2008-11-190520 IS - 23 JF - Blood EP - 5877 TI - Comparative ability of IL-12 and IL-28B to regulate Treg populations and enhance adaptive cellular immunity VL - 113 SP - 5868 KW - adaptive_immunity KW - ifns_type_iii KW - il-12 KW - il-28_inf-lambda3 KW - immune_regulation KW - t_cells KW - treg_cells AU - Morrow, Matthew AU - Pankhong, Panyupa AU - Laddy, Dominick AU - Schoenly, Kimberly AU - Yan, Jian AU - Cisper, Neil AU - Weiner, David PY - 2009/06/04/ UR - http://dx.doi.org/10.1182/blood-2008-11-190520 ER -