Visualizing B cell capture of cognate antigen from follicular dendritic cells Export

@article{citeulike:4785169, abstract = {The prominent display of opsonized antigen by follicular dendritic cells (FDCs) has long favored the view that they serve as antigen-presenting cells for B cells. Surprisingly, however, although B cell capture of antigen from macrophages and dendritic cells has been visualized, acquisition from FDCs has not been directly observed. Using two-photon microscopy, we visualized B cell capture of cognate antigen from FDCs. B cell CXCR5 expression was required, and encounter with FDC-associated antigen could be detected for >1 wk after immunization. B cell-FDC contact times were often brief but occasionally persisted for >30 min, and B cells sometimes acquired antigen together with FDC surface proteins. These observations establish that FDCs can serve as sites of B cell antigen capture, with their prolonged display time ensuring that even rare B cells have the chance of antigen encounter, and they suggest possible information transfer from antigen-presenting cell to B cell. 10.1084/jem.20090209}, author = {Suzuki, Kazuhiro and Grigorova, Irina and Phan, Tri G. and Kelly, Lisa M. and Cyster, Jason G.}, citeulike-article-id = {4785169}, citeulike-linkout-0 = {http://dx.doi.org/10.1084/jem.20090209}, citeulike-linkout-1 = {http://jem.rupress.org/cgi/content/abstract/206/7/1485}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19506051}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19506051}, day = {6}, doi = {10.1084/jem.20090209}, journal = {J. Exp. Med.}, keywords = {adaptive\_immunity, b\_cells, cell\_imaging, dendritic\_cells}, month = {July}, number = {7}, pages = {1485--1493}, posted-at = {2009-07-07 18:04:42}, priority = {2}, title = {Visualizing B cell capture of cognate antigen from follicular dendritic cells}, url = {http://dx.doi.org/10.1084/jem.20090209}, volume = {206}, year = {2009} }

TY - JOUR ID - citeulike:4785169 L3 - citeulike-article-id:4785169 N2 - The prominent display of opsonized antigen by follicular dendritic cells (FDCs) has long favored the view that they serve as antigen-presenting cells for B cells. Surprisingly, however, although B cell capture of antigen from macrophages and dendritic cells has been visualized, acquisition from FDCs has not been directly observed. Using two-photon microscopy, we visualized B cell capture of cognate antigen from FDCs. B cell CXCR5 expression was required, and encounter with FDC-associated antigen could be detected for >1 wk after immunization. B cell-FDC contact times were often brief but occasionally persisted for >30 min, and B cells sometimes acquired antigen together with FDC surface proteins. These observations establish that FDCs can serve as sites of B cell antigen capture, with their prolonged display time ensuring that even rare B cells have the chance of antigen encounter, and they suggest possible information transfer from antigen-presenting cell to B cell. 10.1084/jem.20090209 IS - 7 JF - J. Exp. Med. EP - 1493 TI - Visualizing B cell capture of cognate antigen from follicular dendritic cells VL - 206 SP - 1485 KW - adaptive_immunity KW - b_cells KW - cell_imaging KW - dendritic_cells AU - Suzuki, Kazuhiro AU - Grigorova, Irina AU - Phan, Tri AU - Kelly, Lisa AU - Cyster, Jason PY - 2009/07/06/ UR - http://dx.doi.org/10.1084/jem.20090209 ER -