Population context determines cell-to-cell variability in endocytosis and virus infection Export

@article{citeulike:5658956, abstract = {Single-cell heterogeneity in cell populations arises from a combination of intrinsic and extrinsic factors1, 2, 3. This heterogeneity has been measured for gene transcription, phosphorylation, cell morphology and drug perturbations, and used to explain various aspects of cellular physiology4, 5, 6. In all cases, however, the causes of heterogeneity were not studied. Here we analyse, for the first time, the heterogeneous patterns of related cellular activities, namely virus infection, endocytosis and membrane lipid composition in adherent human cells. We reveal correlations with specific cellular states that are defined by the population context of a cell, and we derive probabilistic models that can explain and predict most cellular heterogeneity of these activities, solely on the basis of each cell's population context. We find that accounting for population-determined heterogeneity is essential for interpreting differences between the activity levels of cell populations. Finally, we reveal that synergy between two molecular components, focal adhesion kinase and the sphingolipid GM1, enhances the population-determined pattern of simian virus 40 (SV40) infection. Our findings provide an explanation for the origin of heterogeneity patterns of cellular activities in adherent cell populations.}, author = {Snijder, Berend and Sacher, Raphael and Ramo, Pauli and Damm, Eva-Maria and Liberali, Prisca and Pelkmans, Lucas}, citeulike-article-id = {5658956}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nature08282}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nature08282}, day = {26}, doi = {10.1038/nature08282}, issn = {0028-0836}, journal = {Nature}, keywords = {cell\_biology, cell\_plasticity, cell\_populations, heterogeneity, viruses}, month = {August}, number = {7263}, pages = {520--523}, posted-at = {2009-09-23 22:07:22}, priority = {2}, publisher = {Nature Publishing Group}, title = {Population context determines cell-to-cell variability in endocytosis and virus infection}, url = {http://dx.doi.org/10.1038/nature08282}, volume = {461}, year = {2009} }

TY - JOUR ID - citeulike:5658956 L3 - citeulike-article-id:5658956 N2 - Single-cell heterogeneity in cell populations arises from a combination of intrinsic and extrinsic factors1, 2, 3. This heterogeneity has been measured for gene transcription, phosphorylation, cell morphology and drug perturbations, and used to explain various aspects of cellular physiology4, 5, 6. In all cases, however, the causes of heterogeneity were not studied. Here we analyse, for the first time, the heterogeneous patterns of related cellular activities, namely virus infection, endocytosis and membrane lipid composition in adherent human cells. We reveal correlations with specific cellular states that are defined by the population context of a cell, and we derive probabilistic models that can explain and predict most cellular heterogeneity of these activities, solely on the basis of each cell's population context. We find that accounting for population-determined heterogeneity is essential for interpreting differences between the activity levels of cell populations. Finally, we reveal that synergy between two molecular components, focal adhesion kinase and the sphingolipid GM1, enhances the population-determined pattern of simian virus 40 (SV40) infection. Our findings provide an explanation for the origin of heterogeneity patterns of cellular activities in adherent cell populations. IS - 7263 JF - Nature SN - 0028-0836 EP - 523 TI - Population context determines cell-to-cell variability in endocytosis and virus infection PB - Nature Publishing Group VL - 461 SP - 520 KW - cell_biology KW - cell_plasticity KW - cell_populations KW - heterogeneity KW - viruses AU - Snijder, Berend AU - Sacher, Raphael AU - Ramo, Pauli AU - Damm, Eva-Maria AU - Liberali, Prisca AU - Pelkmans, Lucas PY - 2009/08/26/ UR - http://dx.doi.org/10.1038/nature08282 ER -