Human T cells express CD25 and FoxP3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function. Export

@article{citeulike:5999758, abstract = {ABSTRACT: BACKGROUND: Foxp3 has been suggested to be a standard marker for murine Tregs whereas its role as marker for human Tregs is controversial. While some reports have shown that human Foxp3+ T cells had no regulatory function others have shown their role in the inhibition of T cell proliferation. Methods: T cell activation was performed by means of brayostatin-1/ionomycin (B/I), mixed lymphocyte reaction (MLR), and CD3/CD28 activation. T cell proliferation was performed using BrdU and CFSE staining. Flow cytometry was performed to determine Foxp3 expression, cell proliferation, viabilities and phenotype analyses of T cells. Results: Both CD4+ and CD8+ T cells expressed Foxp3 upon activation in vitro. Expression of Foxp3 remained more stable in CD4+CD25+ T cells compared to that in CD8+CD25+ T cells. The CD4+CD25+Foxp3+ T cells expressed CD44 and CD62L, showing their effector and memory phenotypes. Both FoxP3- responder T cells and CD4+FoxP3+ T cells underwent proliferation upon CD3/CD28 activation. Conclusion: Expression of Foxp3 does not necessarily convey regulatory function in human CD4+CD25+ T cells. Increased FoxP3 on CD44+ effector and CD44+CD62L+ memory T cells upon stimulation suggest the activation-induced regulation of FoxP3 expression.}, author = {Kmieciak, Maciej and Gowda, Madhu and Graham, Laura and Godder, Kamar and Bear, Harry D. and Marincola, Francesco M. and Manjili, Masoud H.}, citeulike-article-id = {5999758}, citeulike-linkout-0 = {http://dx.doi.org/10.1186/1479-5876-7-89}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19849846}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19849846}, day = {22}, doi = {10.1186/1479-5876-7-89}, issn = {1479-5876}, journal = {Journal of translational medicine}, keywords = {cd25, cell\_plasticity, foxp3, immune\_regulation, treg\_cells}, month = {October}, number = {1}, posted-at = {2009-10-24 14:45:46}, priority = {2}, title = {Human T cells express CD25 and FoxP3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function.}, url = {http://dx.doi.org/10.1186/1479-5876-7-89}, volume = {7}, year = {2009} }

TY - JOUR ID - citeulike:5999758 L3 - citeulike-article-id:5999758 N2 - ABSTRACT: BACKGROUND: Foxp3 has been suggested to be a standard marker for murine Tregs whereas its role as marker for human Tregs is controversial. While some reports have shown that human Foxp3+ T cells had no regulatory function others have shown their role in the inhibition of T cell proliferation. Methods: T cell activation was performed by means of brayostatin-1/ionomycin (B/I), mixed lymphocyte reaction (MLR), and CD3/CD28 activation. T cell proliferation was performed using BrdU and CFSE staining. Flow cytometry was performed to determine Foxp3 expression, cell proliferation, viabilities and phenotype analyses of T cells. Results: Both CD4+ and CD8+ T cells expressed Foxp3 upon activation in vitro. Expression of Foxp3 remained more stable in CD4+CD25+ T cells compared to that in CD8+CD25+ T cells. The CD4+CD25+Foxp3+ T cells expressed CD44 and CD62L, showing their effector and memory phenotypes. Both FoxP3- responder T cells and CD4+FoxP3+ T cells underwent proliferation upon CD3/CD28 activation. Conclusion: Expression of Foxp3 does not necessarily convey regulatory function in human CD4+CD25+ T cells. Increased FoxP3 on CD44+ effector and CD44+CD62L+ memory T cells upon stimulation suggest the activation-induced regulation of FoxP3 expression. IS - 1 JF - Journal of translational medicine SN - 1479-5876 TI - Human T cells express CD25 and FoxP3 upon activation and exhibit effector/memory phenotypes without any regulatory/suppressor function. VL - 7 KW - cd25 KW - cell_plasticity KW - foxp3 KW - immune_regulation KW - treg_cells AU - Kmieciak, Maciej AU - Gowda, Madhu AU - Graham, Laura AU - Godder, Kamar AU - Bear, Harry AU - Marincola, Francesco AU - Manjili, Masoud PY - 2009/10/22/ UR - http://dx.doi.org/10.1186/1479-5876-7-89 ER -