Functional Expression of the Signaling Complex Sensory Rhodopsin II/Transducer II from <i>Halobacterium salinarum</i> in <i>Escherichia coli</i>^&#x2020; Export

@article{citeulike:4937534, abstract = {Sensory rhodopsin II, a photoreceptor from Halobacterium salinarum (HsSRII), in complex with its cognate transducer protein (HsHtrII) triggers the photophobic response via a cytoplasmic two-component signaling cascade. HsHtrII possess in addition to the HsSRII binding and the cytoplasmic domains an extracellular serine-receptor domain. Here we describe the properties of HsSRII and HsHtrII and those of various shortened transducer analogs, heterologously expressed in Escherichia coli. HsSRII displays the photocycle typical of archaeal photosensors with prolonged kinetics. Using an isothermal titration calorimetric analysis for this complex a dissociation constant of 1.1 03BCm was obtained similar to that of the corresponding transducer/receptor pair from Natronobacterium pharaonis. A shortened transducer lacking the extracellular and cytoplasmic domain is also sufficient to bind the receptor with a slightly lower affinity. The dissociation constant of serine binding to the extracellular domain was determined to be about 5 03BCm. This result is in line with the proposal that the extracellular domain indeed is a serine receptor.}, address = {Max-Planck-Institut fr molekulare Physiology, Dortmund, Germany; Medizinische Hochschule Hannover, Institut fr Biophysik. Chemie, Hannover, Germany}, author = {Kim, Young-Jun and Chizhov, Igor and Engelhard, Martin}, citeulike-article-id = {4937534}, citeulike-linkout-0 = {http://dx.doi.org/10.1111/j.1751-1097.2008.00470.x}, citeulike-linkout-1 = {http://www3.interscience.wiley.com/cgi-bin/abstract/121527610/ABSTRACT}, doi = {10.1111/j.1751-1097.2008.00470.x}, journal = {Photochemistry and Photobiology}, number = {2}, pages = {521--528}, posted-at = {2009-06-23 22:48:16}, priority = {2}, title = {Functional Expression of the Signaling Complex Sensory Rhodopsin II/Transducer II from <i>Halobacterium salinarum</i> in <i>Escherichia coli</i>^\&\#x2020;}, url = {http://dx.doi.org/10.1111/j.1751-1097.2008.00470.x}, volume = {85}, year = {2009} }

TY - JOUR ID - citeulike:4937534 L3 - citeulike-article-id:4937534 N2 - Sensory rhodopsin II, a photoreceptor from Halobacterium salinarum (HsSRII), in complex with its cognate transducer protein (HsHtrII) triggers the photophobic response via a cytoplasmic two-component signaling cascade. HsHtrII possess in addition to the HsSRII binding and the cytoplasmic domains an extracellular serine-receptor domain. Here we describe the properties of HsSRII and HsHtrII and those of various shortened transducer analogs, heterologously expressed in Escherichia coli. HsSRII displays the photocycle typical of archaeal photosensors with prolonged kinetics. Using an isothermal titration calorimetric analysis for this complex a dissociation constant of 1.1 03BCm was obtained similar to that of the corresponding transducer/receptor pair from Natronobacterium pharaonis. A shortened transducer lacking the extracellular and cytoplasmic domain is also sufficient to bind the receptor with a slightly lower affinity. The dissociation constant of serine binding to the extracellular domain was determined to be about 5 03BCm. This result is in line with the proposal that the extracellular domain indeed is a serine receptor. IS - 2 JF - Photochemistry and Photobiology AD - Max-Planck-Institut fr molekulare Physiology, Dortmund, Germany; Medizinische Hochschule Hannover, Institut fr Biophysik. Chemie, Hannover, Germany EP - 528 TI - Functional Expression of the Signaling Complex Sensory Rhodopsin II/Transducer II from <i>Halobacterium salinarum</i> in <i>Escherichia coli</i>^&#x2020; VL - 85 SP - 521 AU - Kim, Young-Jun AU - Chizhov, Igor AU - Engelhard, Martin PY - 2009/// UR - http://dx.doi.org/10.1111/j.1751-1097.2008.00470.x ER -