Single-nucleotide-polymorphism typing and genetic relationships of Salmonella enterica serovar Typhi isolates. Export

@article{citeulike:3839316, abstract = {Salmonella enterica serovar Typhi is a clone with a low level of variation. We developed a molecular typing method for serovar Typhi using 38 genome-wide single-nucleotide polymorphisms (SNPs) as markers detected by PCR-restriction enzyme digestion. The 73 worldwide serovar Typhi isolates studied were separated into 23 SNP profiles and four distinct genetic groups. Serovar Typhi isolates expressing the unique flagellar antigen z66 were found to cluster together and branch off from the ancestral group, suggesting that serovar Typhi was initially monophasic with only an H1 antigen and subsequently gained the z66 antigen. Typing using the 38 SNPs gave a discriminatory power of 0.87, and a minimum of 16 SNPs may be used to achieve the same level of differentiation. The SNP typing method we developed will be a valuable tool for global epidemiology studies of serovar Typhi.}, author = {Octavia, S. and Lan, R.}, citeulike-article-id = {3839316}, citeulike-linkout-0 = {http://dx.doi.org/10.1128/JCM.00720-07}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17728466}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17728466}, doi = {10.1128/JCM.00720-07}, issn = {0095-1137}, journal = {Journal of clinical microbiology}, keywords = {genomics, salmonella, snp}, month = {November}, number = {11}, pages = {3795--3801}, posted-at = {2008-12-31 16:37:47}, priority = {2}, title = {Single-nucleotide-polymorphism typing and genetic relationships of Salmonella enterica serovar Typhi isolates.}, url = {http://dx.doi.org/10.1128/JCM.00720-07}, volume = {45}, year = {2007} }

TY - JOUR ID - citeulike:3839316 L3 - citeulike-article-id:3839316 N2 - Salmonella enterica serovar Typhi is a clone with a low level of variation. We developed a molecular typing method for serovar Typhi using 38 genome-wide single-nucleotide polymorphisms (SNPs) as markers detected by PCR-restriction enzyme digestion. The 73 worldwide serovar Typhi isolates studied were separated into 23 SNP profiles and four distinct genetic groups. Serovar Typhi isolates expressing the unique flagellar antigen z66 were found to cluster together and branch off from the ancestral group, suggesting that serovar Typhi was initially monophasic with only an H1 antigen and subsequently gained the z66 antigen. Typing using the 38 SNPs gave a discriminatory power of 0.87, and a minimum of 16 SNPs may be used to achieve the same level of differentiation. The SNP typing method we developed will be a valuable tool for global epidemiology studies of serovar Typhi. IS - 11 JF - Journal of clinical microbiology SN - 0095-1137 EP - 3801 TI - Single-nucleotide-polymorphism typing and genetic relationships of Salmonella enterica serovar Typhi isolates. VL - 45 SP - 3795 KW - genomics KW - salmonella KW - snp AU - Octavia, S AU - Lan, R PY - 2007/11// UR - http://dx.doi.org/10.1128/JCM.00720-07 ER -