Comparison of amplified ribosomal DNA restriction analysis, random amplified polymorphic DNA analysis, and amplified fragment length polymorphism fingerprinting for identification of Acinetobacter genomic species and typing of Acinetobacter baumannii. Export

@article{citeulike:3857481, abstract = {Thirty-one strains of Acinetobacter species, including type strains of the 18 genomic species and 13 clinical isolates, were compared by amplified ribosomal DNA restriction analysis (ARDRA), random amplified polymorphic DNA analysis (RAPD), and amplified fragment length polymorphism (AFLP) fingerprinting. ARDRA, performed with five different enzymes, showed low discriminatory power for differentiating Acinetobacter at the species and strain level. The standardized commercially available RAPD kit clearly enabled the discrimination of all Acinetobacter genomic species but showed great polymorphism between isolates of Acinetobacter baumannii. AFLP fingerprinting with radioactively as well as fluorescently labelled primers showed high discriminatory power for the identification of 18 Acinetobacter genomic species and typing of 13 clinical Acinetobacter isolates. Compared to radioactive AFLP, fluorescent AFLP was technically fast and simple to perform, and it permitted analysis with an automated DNA sequencer. Fluorescent AFLP seems particularly well suited for studying the epidemiology of nosocomial infections and outbreaks caused by Acinetobacter species.}, author = {Koeleman, J. G. and Stoof, J. and Biesmans, D. J. and Savelkoul, P. H. and Vandenbroucke-Grauls, C. M.}, citeulike-article-id = {3857481}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/9705386}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=9705386}, comment = {RAPD looks best, but variability due to differences in primer conctn, DNA quality etc.}, issn = {0095-1137}, journal = {Journal of clinical microbiology}, keywords = {acinetobacter, typing}, month = {September}, number = {9}, pages = {2522--2529}, posted-at = {2009-01-07 14:44:54}, priority = {2}, title = {Comparison of amplified ribosomal DNA restriction analysis, random amplified polymorphic DNA analysis, and amplified fragment length polymorphism fingerprinting for identification of Acinetobacter genomic species and typing of Acinetobacter baumannii.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9705386}, volume = {36}, year = {1998} }

TY - JOUR ID - citeulike:3857481 L3 - citeulike-article-id:3857481 N2 - Thirty-one strains of Acinetobacter species, including type strains of the 18 genomic species and 13 clinical isolates, were compared by amplified ribosomal DNA restriction analysis (ARDRA), random amplified polymorphic DNA analysis (RAPD), and amplified fragment length polymorphism (AFLP) fingerprinting. ARDRA, performed with five different enzymes, showed low discriminatory power for differentiating Acinetobacter at the species and strain level. The standardized commercially available RAPD kit clearly enabled the discrimination of all Acinetobacter genomic species but showed great polymorphism between isolates of Acinetobacter baumannii. AFLP fingerprinting with radioactively as well as fluorescently labelled primers showed high discriminatory power for the identification of 18 Acinetobacter genomic species and typing of 13 clinical Acinetobacter isolates. Compared to radioactive AFLP, fluorescent AFLP was technically fast and simple to perform, and it permitted analysis with an automated DNA sequencer. Fluorescent AFLP seems particularly well suited for studying the epidemiology of nosocomial infections and outbreaks caused by Acinetobacter species. IS - 9 JF - Journal of clinical microbiology SN - 0095-1137 EP - 2529 TI - Comparison of amplified ribosomal DNA restriction analysis, random amplified polymorphic DNA analysis, and amplified fragment length polymorphism fingerprinting for identification of Acinetobacter genomic species and typing of Acinetobacter baumannii. VL - 36 SP - 2522 KW - acinetobacter KW - typing N1 - RAPD looks best, but variability due to differences in primer conctn, DNA quality etc. AU - Koeleman, JG AU - Stoof, J AU - Biesmans, DJ AU - Savelkoul, PH AU - Vandenbroucke-Grauls, CM PY - 1998/09// UR - http://view.ncbi.nlm.nih.gov/pubmed/9705386 ER -