Influence of culture conditions on lipopeptide production by Bacillus subtilis. Export

@article{citeulike:4486373, abstract = {Bacillus subtilis produces various families of lipopeptides with different homologous compounds. To produce "new molecules" with improved activities and to select strains that produced a reduced number of homologs or isomers, we studied the effects of different media on the nature of the synthesis of fatty acid chains for each lipopeptide family. This study focused on two B. subtilis strains cultivated in flasks. Optimized medium for lipopeptide production and Landy medium modified by replacing glutamic acid with other alpha-amino acids were used. We found that the intensity of production of homologous compounds depends on the strain and the culture medium. Analysis of these lipopeptides by high-performance liquid chromatography showed that the strain B. subtilis NT02 yielded various homologous compounds when cultivated in Landy medium (L-Glu), but primarily one homologous product in high relative amounts when cultivated in the optimized medium. Mass spectrometric analysis and determination of the amino acid composition of this molecule enabled us to identify it as Bacillomycine L c15.}, author = {Akpa, E. and Jacques, P. and Wathelet, B. and Paquot, M. and Fuchs, R. and Budzikiewicz, H. and Thonart, P.}, citeulike-article-id = {4486373}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/11963884}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=11963884}, issn = {0273-2289}, journal = {Applied biochemistry and biotechnology}, pages = {551--561}, posted-at = {2009-05-07 12:47:46}, priority = {2}, title = {Influence of culture conditions on lipopeptide production by Bacillus subtilis.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/11963884}, volume = {91-93}, year = {2001} }

TY - JOUR ID - citeulike:4486373 L3 - citeulike-article-id:4486373 N2 - Bacillus subtilis produces various families of lipopeptides with different homologous compounds. To produce "new molecules" with improved activities and to select strains that produced a reduced number of homologs or isomers, we studied the effects of different media on the nature of the synthesis of fatty acid chains for each lipopeptide family. This study focused on two B. subtilis strains cultivated in flasks. Optimized medium for lipopeptide production and Landy medium modified by replacing glutamic acid with other alpha-amino acids were used. We found that the intensity of production of homologous compounds depends on the strain and the culture medium. Analysis of these lipopeptides by high-performance liquid chromatography showed that the strain B. subtilis NT02 yielded various homologous compounds when cultivated in Landy medium (L-Glu), but primarily one homologous product in high relative amounts when cultivated in the optimized medium. Mass spectrometric analysis and determination of the amino acid composition of this molecule enabled us to identify it as Bacillomycine L c15. JF - Applied biochemistry and biotechnology SN - 0273-2289 EP - 561 TI - Influence of culture conditions on lipopeptide production by Bacillus subtilis. VL - 91-93 SP - 551 AU - Akpa, E AU - Jacques, P AU - Wathelet, B AU - Paquot, M AU - Fuchs, R AU - Budzikiewicz, H AU - Thonart, P PY - 2001/// UR - http://view.ncbi.nlm.nih.gov/pubmed/11963884 ER -