Functional interaction of beta-catenin with the transcription factor LEF-1. Export

@article{citeulike:6008852, abstract = {The cytoplasmic proteins beta-catenin of vertebrates and armadillo of Drosophila have two functions: they link the cadherin cell-adhesion molecules to the cytoskeleton, and they participate in the wnt/wingless signal pathway. Here we show, in a yeast two-hybrid screen, that the architectural transcription factor LEF-1 (for lymphoid enhancer-binding factor) interacts with beta-catenin. In mammalian cells, coexpressed LEF-1 and beta-catenin form a complex that is localized to the nucleus and can be detected by immunoprecipitation. Moreover, LEF-1 and beta-catenin form a ternary complex with DNA that splays an altered DNA bend. Microinjection of LEF-1 into XenoPus embryos induces axis duplication, which is augmented by interaction with beta-catenin. Thus beta-catenin regulates gene expression by direct interaction with transcription factors such as LEF-1, providing a molecular mechanism for the transmission of signals, from cell-adhesion components or wnt protein to the nucleus.}, author = {Behrens, J. and von Kries, J. P. and K\"{u}hl, M. and Bruhn, L. and Wedlich, D. and Grosschedl, R. and Birchmeier, W.}, citeulike-article-id = {6008852}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/382638a0}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/8757136}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=8757136}, day = {15}, doi = {10.1038/382638a0}, issn = {0028-0836}, journal = {Nature}, keywords = {mdc}, month = {August}, number = {6592}, pages = {638--642}, posted-at = {2009-10-26 10:35:12}, priority = {2}, title = {Functional interaction of beta-catenin with the transcription factor LEF-1.}, url = {http://dx.doi.org/10.1038/382638a0}, volume = {382}, year = {1996} }

TY - JOUR ID - citeulike:6008852 L3 - citeulike-article-id:6008852 N2 - The cytoplasmic proteins beta-catenin of vertebrates and armadillo of Drosophila have two functions: they link the cadherin cell-adhesion molecules to the cytoskeleton, and they participate in the wnt/wingless signal pathway. Here we show, in a yeast two-hybrid screen, that the architectural transcription factor LEF-1 (for lymphoid enhancer-binding factor) interacts with beta-catenin. In mammalian cells, coexpressed LEF-1 and beta-catenin form a complex that is localized to the nucleus and can be detected by immunoprecipitation. Moreover, LEF-1 and beta-catenin form a ternary complex with DNA that splays an altered DNA bend. Microinjection of LEF-1 into XenoPus embryos induces axis duplication, which is augmented by interaction with beta-catenin. Thus beta-catenin regulates gene expression by direct interaction with transcription factors such as LEF-1, providing a molecular mechanism for the transmission of signals, from cell-adhesion components or wnt protein to the nucleus. IS - 6592 JF - Nature SN - 0028-0836 EP - 642 TI - Functional interaction of beta-catenin with the transcription factor LEF-1. VL - 382 SP - 638 KW - mdc AU - Behrens, J AU - von Kries, JP AU - Kühl, M AU - Bruhn, L AU - Wedlich, D AU - Grosschedl, R AU - Birchmeier, W PY - 1996/08/15/ UR - http://dx.doi.org/10.1038/382638a0 ER -