A Novel CDK9-Associated C-Type Cyclin Interacts Directly with HIV-1 Tat and Mediates Its High-Affinity, Loop-Specific Binding to TAR RNA Export

@article{citeulike:1979041, abstract = {The HIV-1 Tat protein regulates transcription elongation through binding to the viral TAR RNA stem-loop structure. We have isolated a novel 87 kDa cyclin C-related protein (cyclin T) that interacts specifically with the transactivation domain of Tat. Cyclin T is a partner for CDK9, an RNAPII transcription elongation factor. Remarkably, the interaction of Tat with cyclin T strongly enhances the affinity and specificity of the Tat:TAR RNA interaction, and confers a requirement for sequences in the loop of TAR that are not recognized by Tat alone. Moreover, overexpression of human cyclin T rescues Tat activity in nonpermissive rodent cells. We propose that Tat directs cyclin T-CDK9 to RNAPII through cooperative binding to TAR RNA.}, author = {Wei, Ping and Garber, Mitchell E. and Fang, Shi-Min and Fischer, Wolfgang H. and Jones, Katherine A.}, citeulike-article-id = {1979041}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/S0092-8674(00)80939-3}, citeulike-linkout-1 = {http://www.sciencedirect.com/science/article/B6WSN-419K5BX-6/2/7bbfa1ba8362cf85da41a2369f180c14}, day = {20}, doi = {10.1016/S0092-8674(00)80939-3}, journal = {Cell}, keywords = {hiv-1, tar-tat, tat}, month = {February}, number = {4}, pages = {451--462}, posted-at = {2007-11-25 12:12:14}, priority = {2}, title = {A Novel CDK9-Associated C-Type Cyclin Interacts Directly with HIV-1 Tat and Mediates Its High-Affinity, Loop-Specific Binding to TAR RNA}, url = {http://dx.doi.org/10.1016/S0092-8674(00)80939-3}, volume = {92}, year = {1998} }

TY - JOUR ID - citeulike:1979041 L3 - citeulike-article-id:1979041 N2 - The HIV-1 Tat protein regulates transcription elongation through binding to the viral TAR RNA stem-loop structure. We have isolated a novel 87 kDa cyclin C-related protein (cyclin T) that interacts specifically with the transactivation domain of Tat. Cyclin T is a partner for CDK9, an RNAPII transcription elongation factor. Remarkably, the interaction of Tat with cyclin T strongly enhances the affinity and specificity of the Tat:TAR RNA interaction, and confers a requirement for sequences in the loop of TAR that are not recognized by Tat alone. Moreover, overexpression of human cyclin T rescues Tat activity in nonpermissive rodent cells. We propose that Tat directs cyclin T-CDK9 to RNAPII through cooperative binding to TAR RNA. IS - 4 JF - Cell EP - 462 TI - A Novel CDK9-Associated C-Type Cyclin Interacts Directly with HIV-1 Tat and Mediates Its High-Affinity, Loop-Specific Binding to TAR RNA VL - 92 SP - 451 KW - hiv-1 KW - tar-tat KW - tat AU - Wei, Ping AU - Garber, Mitchell AU - Fang, Shi-Min AU - Fischer, Wolfgang AU - Jones, Katherine PY - 1998/02/20/ UR - http://dx.doi.org/10.1016/S0092-8674(00)80939-3 ER -