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Fluorescence Behavioral Imaging (FBI) Tracks Identity in Heterogeneous Groups of Drosophila.

by: Pavan Ramdya, Thomas Schaffter, Dario Floreano, Richard Benton
PloS one, Vol. 7, No. 11. (2012), doi:10.1371/journal.pone.0048381  Key: citeulike:11862619

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Abstract

Distinguishing subpopulations in group behavioral experiments can reveal the impact of differences in genetic, pharmacological and life-histories on social interactions and decision-making. Here we describe Fluorescence Behavioral Imaging (FBI), a toolkit that uses transgenic fluorescence to discriminate subpopulations, imaging hardware that simultaneously records behavior and fluorescence expression, and open-source software for automated, high-accuracy determination of genetic identity. Using FBI, we measure courtship partner choice in genetically mixed groups of Drosophila.


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