A simplified method of generating transgenic Xenopus. Export

@article{citeulike:1531218, abstract = {Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has the potential to increase the numbers of normally developing transgenic embryos.}, author = {Sparrow, D. B. and Latinkic, B. and Mohun, T. J.}, citeulike-article-id = {1531218}, citeulike-linkout-0 = {http://dx.doi.org/10.1093/nar/28.4.e12}, citeulike-linkout-1 = {http://nar.oxfordjournals.org/cgi/content/abstract/28/4/e12}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/10648800}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=10648800}, day = {15}, doi = {10.1093/nar/28.4.e12}, issn = {1362-4962}, journal = {Nucleic acids research}, keywords = {frog, h, transgenic}, month = {February}, number = {4}, pages = {e12+}, posted-at = {2007-08-02 20:50:15}, priority = {3}, title = {A simplified method of generating transgenic Xenopus.}, url = {http://dx.doi.org/10.1093/nar/28.4.e12}, volume = {28}, year = {2000} }

TY - JOUR ID - citeulike:1531218 L3 - citeulike-article-id:1531218 N2 - Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has the potential to increase the numbers of normally developing transgenic embryos. IS - 4 JF - Nucleic acids research SN - 1362-4962 TI - A simplified method of generating transgenic Xenopus. VL - 28 SP - e12 KW - frog KW - h KW - transgenic AU - Sparrow, DB AU - Latinkic, B AU - Mohun, TJ PY - 2000/02/15/ UR - http://dx.doi.org/10.1093/nar/28.4.e12 ER -