The isolated Xenopus laevis tail: a preparation for studying the central nervous system and metamorphosis in culture. Export

@article{citeulike:5971662, abstract = {THE Xenopus tail preparation described here was initially developed for studying the action of substances on the vertebrate nervous system. For this both physiological preparations and whole animals have their disadvantages. The former are short-lived; the latter may die before any major chemical or structural changes have had time to occur. It was therefore thought desirable to seek a relatively long-lived preparation that possessed a functional central nervous system, but yet was not dependent on the conduction of nerve impulses for its survival. The tail of a Xenopus laevis tadpole seemed to be highly suitable for this purpose, as it contained complete reflex arcs and was sufficiently small to carry out its necessary exchanges by diffusion. Moreover, it was so transparent that nerves and muscle striations could be studied microscopically during life. Although agents to which the skin was impermeable could easily be injected into the tail, it was thought best to seek media that would be suitable for partially flayed tails.}, author = {Shaffer, B. M.}, citeulike-article-id = {5971662}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/13988328}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=13988328}, issn = {0022-0752}, journal = {Journal of embryology and experimental morphology}, keywords = {culture\_tail, frog, h, t}, month = {March}, pages = {77--90}, posted-at = {2009-10-19 18:08:13}, priority = {2}, title = {The isolated Xenopus laevis tail: a preparation for studying the central nervous system and metamorphosis in culture.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/13988328}, volume = {11}, year = {1963} }

TY - JOUR ID - citeulike:5971662 L3 - citeulike-article-id:5971662 N2 - THE Xenopus tail preparation described here was initially developed for studying the action of substances on the vertebrate nervous system. For this both physiological preparations and whole animals have their disadvantages. The former are short-lived; the latter may die before any major chemical or structural changes have had time to occur. It was therefore thought desirable to seek a relatively long-lived preparation that possessed a functional central nervous system, but yet was not dependent on the conduction of nerve impulses for its survival. The tail of a Xenopus laevis tadpole seemed to be highly suitable for this purpose, as it contained complete reflex arcs and was sufficiently small to carry out its necessary exchanges by diffusion. Moreover, it was so transparent that nerves and muscle striations could be studied microscopically during life. Although agents to which the skin was impermeable could easily be injected into the tail, it was thought best to seek media that would be suitable for partially flayed tails. JF - Journal of embryology and experimental morphology SN - 0022-0752 EP - 90 TI - The isolated Xenopus laevis tail: a preparation for studying the central nervous system and metamorphosis in culture. VL - 11 SP - 77 KW - culture_tail KW - frog KW - h KW - t AU - Shaffer, BM PY - 1963/03// UR - http://view.ncbi.nlm.nih.gov/pubmed/13988328 ER -