Role of hemoglobin in improving biodegradation of aromatic contaminants under hypoxic conditions. Export

@article{citeulike:5842192, abstract = {Genetic engineering of bacteria using the Vitreoscilla (bacterial) hemoglobin gene has been used to enhance bioremediation of several compounds which are models for, or are themselves, toxic chemicals which may contaminate soil and water. Initial experiments, done mostly in shake flasks, with Escherichia coli, Burkholderia sp. DNT and Pseudomonas aeruginosa demonstrated that expression of Vitreoscilla hemoglobin in heterologous hosts can enhance biodegradation of several aromatic compounds as well as an organophosphorus compound. These studies concentrated for the most part on enhancement of endogenous catabolic capabilities of the hosts; the presence of vgb/VHb enhanced both growth and biodegradation. The initial studies were followed by experiments in systems which more closely approximated conditions that would exist in field applications. These included soil columns, continuous flow reactors and membrane bioreactors. The latter work also enabled calculation of the effects of the presence of vgb/VHb on kinetic parameters such as growth rate, substrate and oxygen utilization rate, and degradation rate of pollutants, etc. Although not always the case, for the most part, and particularly in bioreactors, the advantages due to vgb/VHb were greater under conditions of limited aeration or hypoxic conditions.}, author = {Stark, Benjamin C. and Urgun-Demirtas, Meltem and Pagilla, Krishna R.}, citeulike-article-id = {5842192}, citeulike-linkout-0 = {http://www.biomedexperts.com/Abstract.bme/18685270}, citeulike-linkout-1 = {http://dx.doi.org/10.1159/000121329}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18685270}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18685270}, doi = {10.1159/000121329}, issn = {1660-2412}, journal = {Journal of molecular microbiology and biotechnology}, keywords = {aromatic, biodegradation, conditions, contaminants, hemoglobin, hypoxic, improving, in, of, role, under}, number = {2-3}, pages = {181--189}, posted-at = {2009-09-26 08:23:05}, priority = {4}, title = {Role of hemoglobin in improving biodegradation of aromatic contaminants under hypoxic conditions.}, url = {http://dx.doi.org/10.1159/000121329}, volume = {15}, year = {2008} }

TY - JOUR ID - citeulike:5842192 L3 - citeulike-article-id:5842192 N2 - Genetic engineering of bacteria using the Vitreoscilla (bacterial) hemoglobin gene has been used to enhance bioremediation of several compounds which are models for, or are themselves, toxic chemicals which may contaminate soil and water. Initial experiments, done mostly in shake flasks, with Escherichia coli, Burkholderia sp. DNT and Pseudomonas aeruginosa demonstrated that expression of Vitreoscilla hemoglobin in heterologous hosts can enhance biodegradation of several aromatic compounds as well as an organophosphorus compound. These studies concentrated for the most part on enhancement of endogenous catabolic capabilities of the hosts; the presence of vgb/VHb enhanced both growth and biodegradation. The initial studies were followed by experiments in systems which more closely approximated conditions that would exist in field applications. These included soil columns, continuous flow reactors and membrane bioreactors. The latter work also enabled calculation of the effects of the presence of vgb/VHb on kinetic parameters such as growth rate, substrate and oxygen utilization rate, and degradation rate of pollutants, etc. Although not always the case, for the most part, and particularly in bioreactors, the advantages due to vgb/VHb were greater under conditions of limited aeration or hypoxic conditions. IS - 2-3 JF - Journal of molecular microbiology and biotechnology SN - 1660-2412 EP - 189 TI - Role of hemoglobin in improving biodegradation of aromatic contaminants under hypoxic conditions. VL - 15 SP - 181 KW - aromatic KW - biodegradation KW - conditions KW - contaminants KW - hemoglobin KW - hypoxic KW - improving KW - in KW - of KW - role KW - under AU - Stark, Benjamin AU - Urgun-Demirtas, Meltem AU - Pagilla, Krishna PY - 2008/// UR - http://dx.doi.org/10.1159/000121329 ER -