Studying protein dynamics in living cells. Export

@article{citeulike:675320, abstract = {Since the advent of the green fluorescent protein, the subcellular localization, mobility, transport routes and binding interactions of proteins can be studied in living cells. Live cell imaging, in combination with photobleaching, energy transfer or fluorescence correlation spectroscopy are providing unprecedented insights into the movement of proteins and their interactions with cellular components. Remarkably, these powerful techniques are accessible to non-specialists using commercially available microscope systems.}, address = {Cell Biology and Metabolism Branch, 18 Library Drive, NICHD, NIH Bethesda, Maryland 20892-5430 USA. jlippin@helix.nih.gov}, author = {Lippincott-Schwartz, J. and Snapp, E. and Kenworthy, A.}, citeulike-article-id = {675320}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/35073068}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/11389468}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=11389468}, doi = {10.1038/35073068}, issn = {1471-0072}, journal = {Nat Rev Mol Cell Biol}, keywords = {frap, fret}, month = {June}, number = {6}, pages = {444--456}, posted-at = {2006-05-30 19:32:23}, priority = {0}, title = {Studying protein dynamics in living cells.}, url = {http://dx.doi.org/10.1038/35073068}, volume = {2}, year = {2001} }

TY - JOUR ID - citeulike:675320 L3 - citeulike-article-id:675320 N2 - Since the advent of the green fluorescent protein, the subcellular localization, mobility, transport routes and binding interactions of proteins can be studied in living cells. Live cell imaging, in combination with photobleaching, energy transfer or fluorescence correlation spectroscopy are providing unprecedented insights into the movement of proteins and their interactions with cellular components. Remarkably, these powerful techniques are accessible to non-specialists using commercially available microscope systems. IS - 6 JF - Nat Rev Mol Cell Biol SN - 1471-0072 AD - Cell Biology and Metabolism Branch, 18 Library Drive, NICHD, NIH Bethesda, Maryland 20892-5430 USA. jlippin@helix.nih.gov EP - 456 TI - Studying protein dynamics in living cells. VL - 2 SP - 444 KW - frap KW - fret AU - Lippincott-Schwartz, J AU - Snapp, E AU - Kenworthy, A PY - 2001/06// UR - http://dx.doi.org/10.1038/35073068 ER -