Identification of melatonin-regulated genes in the ovine pituitary pars tuberalis, a target site for seasonal hormone control. Export

@article{citeulike:3171023, abstract = {The pars tuberalis (PT) of the pituitary gland expresses a high density of melatonin (MEL) receptors and is believed to regulate seasonal physiology by decoding changes in nocturnal melatonin secretion. Circadian clock genes are known to be expressed in the PT in response to the decline (Per1) and onset (Cry1) of MEL secretion, but to date little is known of other molecular changes in this key MEL target site. To identify transcriptional pathways that may be involved in the diurnal and photoperiod-transduction mechanism, we performed a whole genome transcriptome analysis using PT RNA isolated from sheep culled at three time points over the 24h cycle under either long or short photoperiods. Our results reveal 153 transcripts where expression differs between photoperiods at the light/dark transition and 54 transcripts where expression level was more globally altered by photoperiod (all time points combined). Cry1 induction at night was associated with up-regulation of genes coding for NeuroD1 (Neurogenic differentiation factor 1), Pbef/Nampt, (nicotinamide phosphoribosyltransferase), Hif1alpha (hypoxia-inducible factor-1alpha) and Kcnq5 (K(+) channel) and down-regulation of Rorbeta, a key clock gene regulator. Using in situ hybridization, we confirmed day-night differences in expression for Pbef/Nampt, NeuroD1 and Rorbeta in the PT. Treatment of sheep with MEL increased PT expression for Cry1, Pbef/ Nampt, NeuroD1 and Hif1alpha, but not Kcnq5. Our data thus reveal a cluster of Cry1 associated genes which are acutely responsive to MEL and novel transcriptional pathways involved in MEL action in the PT.}, address = {University of Manchester, Faculty of Life Sciences and Faculty of Medical and Human Sciences, Manchester, UK; University of Edinburgh, Roslin Institute, Midlothian, UK; Physiologie de la Reproduction et des Comportements, UMR INRA-CNRS-University of Tours-Haras Nationaux, 37380 Nouzilly, France; University of Edinburgh, Centre for Reproductive Biology, QMRI, Little France Crescent, Edinburgh, UK.}, author = {Dupr\'{e}, Sandrine M M. and Burt, Dave W W. and Talbot, Richard and Downing, Alison and Mouzaki, Daphne and Waddington, David and Malpaux, Benoit and Davis, Julian Re R. and Lincoln, Gerald A A. and Loudon, Andrew Si S.}, citeulike-article-id = {3171023}, citeulike-linkout-0 = {http://dx.doi.org/10.1210/en.2008-0834}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18669596}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18669596}, day = {31}, doi = {10.1210/en.2008-0834}, issn = {0013-7227}, journal = {Endocrinology}, keywords = {circadian, melatonin, photoperiod}, month = {July}, posted-at = {2008-08-29 04:16:15}, priority = {3}, title = {Identification of melatonin-regulated genes in the ovine pituitary pars tuberalis, a target site for seasonal hormone control.}, url = {http://dx.doi.org/10.1210/en.2008-0834}, year = {2008} }

TY - JOUR ID - citeulike:3171023 L3 - citeulike-article-id:3171023 N2 - The pars tuberalis (PT) of the pituitary gland expresses a high density of melatonin (MEL) receptors and is believed to regulate seasonal physiology by decoding changes in nocturnal melatonin secretion. Circadian clock genes are known to be expressed in the PT in response to the decline (Per1) and onset (Cry1) of MEL secretion, but to date little is known of other molecular changes in this key MEL target site. To identify transcriptional pathways that may be involved in the diurnal and photoperiod-transduction mechanism, we performed a whole genome transcriptome analysis using PT RNA isolated from sheep culled at three time points over the 24h cycle under either long or short photoperiods. Our results reveal 153 transcripts where expression differs between photoperiods at the light/dark transition and 54 transcripts where expression level was more globally altered by photoperiod (all time points combined). Cry1 induction at night was associated with up-regulation of genes coding for NeuroD1 (Neurogenic differentiation factor 1), Pbef/Nampt, (nicotinamide phosphoribosyltransferase), Hif1alpha (hypoxia-inducible factor-1alpha) and Kcnq5 (K(+) channel) and down-regulation of Rorbeta, a key clock gene regulator. Using in situ hybridization, we confirmed day-night differences in expression for Pbef/Nampt, NeuroD1 and Rorbeta in the PT. Treatment of sheep with MEL increased PT expression for Cry1, Pbef/ Nampt, NeuroD1 and Hif1alpha, but not Kcnq5. Our data thus reveal a cluster of Cry1 associated genes which are acutely responsive to MEL and novel transcriptional pathways involved in MEL action in the PT. JF - Endocrinology SN - 0013-7227 AD - University of Manchester, Faculty of Life Sciences and Faculty of Medical and Human Sciences, Manchester, UK; University of Edinburgh, Roslin Institute, Midlothian, UK; Physiologie de la Reproduction et des Comportements, UMR INRA-CNRS-University of Tours-Haras Nationaux, 37380 Nouzilly, France; University of Edinburgh, Centre for Reproductive Biology, QMRI, Little France Crescent, Edinburgh, UK. TI - Identification of melatonin-regulated genes in the ovine pituitary pars tuberalis, a target site for seasonal hormone control. KW - circadian KW - melatonin KW - photoperiod AU - Dupré, Sandrine M AU - Burt, Dave W AU - Talbot, Richard AU - Downing, Alison AU - Mouzaki, Daphne AU - Waddington, David AU - Malpaux, Benoit AU - Davis, Julian Re AU - Lincoln, Gerald A AU - Loudon, Andrew Si PY - 2008/07/31/ UR - http://dx.doi.org/10.1210/en.2008-0834 ER -