Vasoactive intestinal peptide inhibits toll-like receptor 3-induced nitric oxide production in Schwann cells and subsequent sensory neuronal cell death in vitro. Export

@article{citeulike:3171028, abstract = {We have previously reported that polyinosinic-polycytidylic acids [poly(I:C)], a synthetic toll-like receptor 3 (TLR3) agonist, induce Schwann cell activation, which exerts neurotoxic effects on sensory neurons. In this study, we investigated the effects of vasoactive intestinal peptide (VIP), a neuropeptide implicated in nerve regeneration, on TLR3-induced Schwann cell activation. VIP receptors VPAC1 and VPAC2 were constitutively expressed in rat Schwann cells. VIP pretreatment inhibited TLR3-induced inducible nitric oxide synthase (iNOS) gene expression and NO production in Schwann cells. Studies on the intracellular signal transduction pathways indicate that the VIP effect is mediated by protein kinase A activation. VIP also inhibited the poly(I:C)-induced p38 activation that is responsible for the iNOS gene expression in Schwann cells. Finally, VIP inhibited dorsal rooyt ganglion neuronal cell death caused by NO produced in activated Schwann cells. Taken together, our data suggest that VIP exerts a neuroprotective effect by inhibiting neurotoxic Schwann cell activation. (c) 2008 Wiley-Liss, Inc.}, address = {Program in Molecular and Cellular Neuroscience, DRI, and Department of Oral Physiology, School of Dentistry, Seoul National University, Seoul, Korea.}, author = {Lee, Hyunkyoung and Park, Kyungpyo and Kim, Joong Soo S. and Lee, Sung Joong J.}, citeulike-article-id = {3171028}, citeulike-linkout-0 = {http://dx.doi.org/10.1002/jnr.21820}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18683246}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18683246}, day = {5}, doi = {10.1002/jnr.21820}, issn = {1097-4547}, journal = {Journal of neuroscience research}, keywords = {inos, neuroprotection, no, schwann\_cells, tlr3, vip}, month = {August}, posted-at = {2008-08-29 04:26:28}, priority = {3}, title = {Vasoactive intestinal peptide inhibits toll-like receptor 3-induced nitric oxide production in Schwann cells and subsequent sensory neuronal cell death in vitro.}, url = {http://dx.doi.org/10.1002/jnr.21820}, year = {2008} }

TY - JOUR ID - citeulike:3171028 L3 - citeulike-article-id:3171028 N2 - We have previously reported that polyinosinic-polycytidylic acids [poly(I:C)], a synthetic toll-like receptor 3 (TLR3) agonist, induce Schwann cell activation, which exerts neurotoxic effects on sensory neurons. In this study, we investigated the effects of vasoactive intestinal peptide (VIP), a neuropeptide implicated in nerve regeneration, on TLR3-induced Schwann cell activation. VIP receptors VPAC1 and VPAC2 were constitutively expressed in rat Schwann cells. VIP pretreatment inhibited TLR3-induced inducible nitric oxide synthase (iNOS) gene expression and NO production in Schwann cells. Studies on the intracellular signal transduction pathways indicate that the VIP effect is mediated by protein kinase A activation. VIP also inhibited the poly(I:C)-induced p38 activation that is responsible for the iNOS gene expression in Schwann cells. Finally, VIP inhibited dorsal rooyt ganglion neuronal cell death caused by NO produced in activated Schwann cells. Taken together, our data suggest that VIP exerts a neuroprotective effect by inhibiting neurotoxic Schwann cell activation. (c) 2008 Wiley-Liss, Inc. JF - Journal of neuroscience research SN - 1097-4547 AD - Program in Molecular and Cellular Neuroscience, DRI, and Department of Oral Physiology, School of Dentistry, Seoul National University, Seoul, Korea. TI - Vasoactive intestinal peptide inhibits toll-like receptor 3-induced nitric oxide production in Schwann cells and subsequent sensory neuronal cell death in vitro. KW - inos KW - neuroprotection KW - no KW - schwann_cells KW - tlr3 KW - vip AU - Lee, Hyunkyoung AU - Park, Kyungpyo AU - Kim, Joong Soo AU - Lee, Sung Joong PY - 2008/08/05/ UR - http://dx.doi.org/10.1002/jnr.21820 ER -