Estrogen directly modulates circadian rhythms of PER2 expression in the uterus. Export

@article{citeulike:3179826, abstract = {Fluctuations in circulating estrogen and progesterone levels associated with the estrous cycle alter circadian rhythms of physiology and behavior in female rodents. Endogenously applied estrogen shortens the period of the locomotor activity rhythm in rodents. We recently found that estrogen implants affect Period (Per) gene expression in the suprachiasmatic nucleus (SCN; central clock) and uterus of rats in vivo. To explore whether estrogen directly influences the circadian clock in the SCN and/or tissues of the reproductive system, we examined the effects of 17beta-estradiol (E2) on PER2::LUCIFERASE (PER2::LUC) expression in tissue explant cultures from ovariectomized PER2::LUC knockin mice. E2 applied to explanted cultures shortened the period of rhythmic PER2::LUC expression in the uterus, but did not change the period of PER2::LUC expression in the SCN. Raloxifene, a selective estrogen receptor modulator (SERM) and known E2 antagonist in uterine tissues, attenuated the effect of E2 on the period of the PER2::LUC rhythm in the uterus. These data indicate that estrogen directly affects the timing of the molecular clock in the uterus via an estrogen receptor-mediated response. Key words: Ovarian steroid hormone, PER2::LUCIFERASE, Raloxifene, Period.}, address = {University of California Los Angeles.}, author = {Nakamura, Takahiro J J. and Sellix, Michael T T. and Menaker, Michael and Block, Gene D D.}, citeulike-article-id = {3179826}, citeulike-linkout-0 = {http://dx.doi.org/10.1152/ajpendo.90392.2008}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/18728223}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=18728223}, day = {26}, doi = {10.1152/ajpendo.90392.2008}, issn = {0193-1849}, journal = {American journal of physiology. Endocrinology and metabolism}, keywords = {circadian, estrogen, per2, uterus}, month = {August}, posted-at = {2008-09-01 20:47:16}, priority = {4}, title = {Estrogen directly modulates circadian rhythms of PER2 expression in the uterus.}, url = {http://dx.doi.org/10.1152/ajpendo.90392.2008}, year = {2008} }

TY - JOUR ID - citeulike:3179826 L3 - citeulike-article-id:3179826 N2 - Fluctuations in circulating estrogen and progesterone levels associated with the estrous cycle alter circadian rhythms of physiology and behavior in female rodents. Endogenously applied estrogen shortens the period of the locomotor activity rhythm in rodents. We recently found that estrogen implants affect Period (Per) gene expression in the suprachiasmatic nucleus (SCN; central clock) and uterus of rats in vivo. To explore whether estrogen directly influences the circadian clock in the SCN and/or tissues of the reproductive system, we examined the effects of 17beta-estradiol (E2) on PER2::LUCIFERASE (PER2::LUC) expression in tissue explant cultures from ovariectomized PER2::LUC knockin mice. E2 applied to explanted cultures shortened the period of rhythmic PER2::LUC expression in the uterus, but did not change the period of PER2::LUC expression in the SCN. Raloxifene, a selective estrogen receptor modulator (SERM) and known E2 antagonist in uterine tissues, attenuated the effect of E2 on the period of the PER2::LUC rhythm in the uterus. These data indicate that estrogen directly affects the timing of the molecular clock in the uterus via an estrogen receptor-mediated response. Key words: Ovarian steroid hormone, PER2::LUCIFERASE, Raloxifene, Period. JF - American journal of physiology. Endocrinology and metabolism SN - 0193-1849 AD - University of California Los Angeles. TI - Estrogen directly modulates circadian rhythms of PER2 expression in the uterus. KW - circadian KW - estrogen KW - per2 KW - uterus AU - Nakamura, Takahiro J AU - Sellix, Michael T AU - Menaker, Michael AU - Block, Gene D PY - 2008/08/26/ UR - http://dx.doi.org/10.1152/ajpendo.90392.2008 ER -