Quantitative discrimination between endogenousSHG sources in mammalian tissue, based ontheir polarization response Export

@article{citeulike:6087663, abstract = {In this study, the second harmonic generation (SHG) response to polarization and subsequent data analysis is used to discriminate, in the same image, different SHG source architectures with pixel resolution. This is demonstrated in a mammalian tissue containing both skeletal muscle and fibrilar collagen. The SHG intensity variation with the input polarization (PSHG) is fitted pixel by pixel in the image using an algorithm based on a generalized biophysical model. The analysis provides the effective orientation, θe, of the different SHG active structures (harmonophores) at every pixel. This results in a new image in which collagen and muscle are clearly differentiated. In order to quantify the SHG response, the distribution of θe for every harmonophore is obtained. We found that for collagen, the distribution was centered at θe = 42.7° with a full width at half maximum of ∆θ = 5.9° while for muscle θe = 65.3°, with ∆θ = 7.7°. By comparing these distributions, a quantitative measurement of the discrimination procedure is provided.}, author = {Psilodimitrakopoulos, Sotiris and Artigas, David and Soria, Guadalupe and Amat-Roldan, Ivan and Planas, Anna M. and Loza-Alvarez, Pablo}, citeulike-article-id = {6087663}, citeulike-linkout-0 = {http://dx.doi.org/10.1364/OE.17.010168}, citeulike-linkout-1 = {http://www.opticsinfobase.org/abstract.cfm?id=180341}, day = {8}, doi = {10.1364/OE.17.010168}, journal = {Opt. Express}, keywords = {myosin, shg}, month = {June}, number = {12}, pages = {10168--10176}, posted-at = {2009-11-09 11:18:46}, priority = {2}, publisher = {OSA}, title = {Quantitative discrimination between endogenousSHG sources in mammalian tissue, based ontheir polarization response}, url = {http://dx.doi.org/10.1364/OE.17.010168}, volume = {17}, year = {2009} }

TY - JOUR ID - citeulike:6087663 L3 - citeulike-article-id:6087663 N2 - In this study, the second harmonic generation (SHG) response to polarization and subsequent data analysis is used to discriminate, in the same image, different SHG source architectures with pixel resolution. This is demonstrated in a mammalian tissue containing both skeletal muscle and fibrilar collagen. The SHG intensity variation with the input polarization (PSHG) is fitted pixel by pixel in the image using an algorithm based on a generalized biophysical model. The analysis provides the effective orientation, θe, of the different SHG active structures (harmonophores) at every pixel. This results in a new image in which collagen and muscle are clearly differentiated. In order to quantify the SHG response, the distribution of θe for every harmonophore is obtained. We found that for collagen, the distribution was centered at θe = 42.7° with a full width at half maximum of ∆θ = 5.9° while for muscle θe = 65.3°, with ∆θ = 7.7°. By comparing these distributions, a quantitative measurement of the discrimination procedure is provided. IS - 12 JF - Opt. Express EP - 10176 TI - Quantitative discrimination between endogenousSHG sources in mammalian tissue, based ontheir polarization response PB - OSA VL - 17 SP - 10168 KW - myosin KW - shg AU - Psilodimitrakopoulos, Sotiris AU - Artigas, David AU - Soria, Guadalupe AU - Amat-Roldan, Ivan AU - Planas, Anna AU - Loza-Alvarez, Pablo PY - 2009/06/08/ UR - http://dx.doi.org/10.1364/OE.17.010168 ER -