Chiroptical Effects in the Second Harmonic Signal of Collagens I and IV Export

@article{citeulike:6088455, abstract = {PMID: 16028943 We performed polarization-resolved surface second harmonic generation (SHG) experiments on thin films of collagen I and IV molecules, as well as conventional CD measurements. We found that collagen IV presents little CD and no SHG optical activity, whereas collagen I exhibits large chiroptical effects involving both one-electron and excitonic coupling mechanisms. We estimated that these chiral components enhance the SHG signal from fibrillar collagen in biological tissues by typically a factor of 2. By comparing the distinct behaviors of collagens I and IV in SHG microscopy and in surface SHG experiments, we concluded that SHG microscopy is a sensitive probe of the micrometer-scale structural organization of collagen in biological tissues.}, author = {Pena, Ana-Maria and Boulesteix, Thierry and Dartigalongue, Thibault and Schanne-Klein, Marie-Claire}, citeulike-article-id = {6088455}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/ja0520969}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/ja0520969}, day = {1}, doi = {10.1021/ja0520969}, journal = {Journal of the American Chemical Society}, keywords = {collagen, shg}, month = {July}, number = {29}, pages = {10314--10322}, posted-at = {2009-11-09 14:12:37}, priority = {2}, title = {Chiroptical Effects in the Second Harmonic Signal of Collagens I and IV}, url = {http://dx.doi.org/10.1021/ja0520969}, volume = {127}, year = {2005} }

TY - JOUR ID - citeulike:6088455 L3 - citeulike-article-id:6088455 N2 - PMID: 16028943 We performed polarization-resolved surface second harmonic generation (SHG) experiments on thin films of collagen I and IV molecules, as well as conventional CD measurements. We found that collagen IV presents little CD and no SHG optical activity, whereas collagen I exhibits large chiroptical effects involving both one-electron and excitonic coupling mechanisms. We estimated that these chiral components enhance the SHG signal from fibrillar collagen in biological tissues by typically a factor of 2. By comparing the distinct behaviors of collagens I and IV in SHG microscopy and in surface SHG experiments, we concluded that SHG microscopy is a sensitive probe of the micrometer-scale structural organization of collagen in biological tissues. IS - 29 JF - Journal of the American Chemical Society EP - 10322 TI - Chiroptical Effects in the Second Harmonic Signal of Collagens I and IV VL - 127 SP - 10314 KW - collagen KW - shg AU - Pena, Ana-Maria AU - Boulesteix, Thierry AU - Dartigalongue, Thibault AU - Schanne-Klein, Marie-Claire PY - 2005/07/01/ UR - http://dx.doi.org/10.1021/ja0520969 ER -