Uniform polarity microtubule assemblies imaged in native brain tissue by second-harmonic generation microscopy Export

@article{citeulike:6093612, abstract = {10.1073/pnas.0731953100 Microtubule (MT) ensemble polarity is a diagnostic determinant of the structure and function of neuronal processes. Here, polarized MT structures are selectively imaged with second-harmonic generation (SHG) microscopy in native brain tissue. This SHG is found to colocalize with axons in both brain slices and cultured neurons. Because SHG arises only from noninversion symmetric structures, the uniform polarity of axonal MTs leads to the observed signal, whereas the mixed polarity in dendrites leads to destructive interference. SHG imaging provides a tool to investigate the kinetics and function of MT ensemble polarity in dynamic native brain tissue structures and other subcellular motility structures based on polarized MTs.}, author = {Dombeck, Daniel A. and Kasischke, Karl A. and Vishwasrao, Harshad D. and Ingelsson, Martin and Hyman, Bradley T. and Webb, Watt W.}, citeulike-article-id = {6093612}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0731953100}, citeulike-linkout-1 = {http://www.pnas.org/content/100/12/7081.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/100/12/7081.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/12766225}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=12766225}, day = {10}, doi = {10.1073/pnas.0731953100}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {shg, tubulin}, month = {June}, number = {12}, pages = {7081--7086}, posted-at = {2009-11-10 13:57:32}, priority = {2}, title = {Uniform polarity microtubule assemblies imaged in native brain tissue by second-harmonic generation microscopy}, url = {http://dx.doi.org/10.1073/pnas.0731953100}, volume = {100}, year = {2003} }

TY - JOUR ID - citeulike:6093612 L3 - citeulike-article-id:6093612 N2 - 10.1073/pnas.0731953100 Microtubule (MT) ensemble polarity is a diagnostic determinant of the structure and function of neuronal processes. Here, polarized MT structures are selectively imaged with second-harmonic generation (SHG) microscopy in native brain tissue. This SHG is found to colocalize with axons in both brain slices and cultured neurons. Because SHG arises only from noninversion symmetric structures, the uniform polarity of axonal MTs leads to the observed signal, whereas the mixed polarity in dendrites leads to destructive interference. SHG imaging provides a tool to investigate the kinetics and function of MT ensemble polarity in dynamic native brain tissue structures and other subcellular motility structures based on polarized MTs. IS - 12 JF - Proceedings of the National Academy of Sciences of the United States of America EP - 7086 TI - Uniform polarity microtubule assemblies imaged in native brain tissue by second-harmonic generation microscopy VL - 100 SP - 7081 KW - shg KW - tubulin AU - Dombeck, Daniel AU - Kasischke, Karl AU - Vishwasrao, Harshad AU - Ingelsson, Martin AU - Hyman, Bradley AU - Webb, Watt PY - 2003/06/10/ UR - http://dx.doi.org/10.1073/pnas.0731953100 ER -