Second-harmonic microscopy of unstained living cardiacmyocytes:?measurements of sarcomere length with 20-nmaccuracy Export

@article{citeulike:6094955, abstract = {We extend second-harmonic generation (SHG) microscopy to the measurement of sarcomere length in unstained living cardiac myocytes with 20-nm accuracy. We quantify individual sarcomere shortening in the presence of saxitoxin and find that it is in agreement with mechanical measurements of atrial tissue contracture. This functional application of SHG microscopy is generally applicable to quantify the physiological effects of drugs on contractile tissue. Our data also suggest that packed myosin heads in sarcomere thick filaments are responsible for the large second-harmonic endogenous signal in muscle tissue.}, author = {Boulesteix, Thierry and Beaurepaire, Emmanuel and Sauviat, Martin-Pierre and Schanne-Klein, Marie-Claire}, citeulike-article-id = {6094955}, citeulike-linkout-0 = {http://dx.doi.org/10.1364/OL.29.002031}, citeulike-linkout-1 = {http://www.opticsinfobase.org/abstract.cfm?id=80997}, day = {1}, doi = {10.1364/OL.29.002031}, journal = {Opt. Lett.}, keywords = {sarcomere, shg}, month = {September}, number = {17}, pages = {2031--2033}, posted-at = {2009-11-10 14:30:42}, priority = {2}, publisher = {OSA}, title = {Second-harmonic microscopy of unstained living cardiacmyocytes:?measurements of sarcomere length with 20-nmaccuracy}, url = {http://dx.doi.org/10.1364/OL.29.002031}, volume = {29}, year = {2004} }

TY - JOUR ID - citeulike:6094955 L3 - citeulike-article-id:6094955 N2 - We extend second-harmonic generation (SHG) microscopy to the measurement of sarcomere length in unstained living cardiac myocytes with 20-nm accuracy. We quantify individual sarcomere shortening in the presence of saxitoxin and find that it is in agreement with mechanical measurements of atrial tissue contracture. This functional application of SHG microscopy is generally applicable to quantify the physiological effects of drugs on contractile tissue. Our data also suggest that packed myosin heads in sarcomere thick filaments are responsible for the large second-harmonic endogenous signal in muscle tissue. IS - 17 JF - Opt. Lett. EP - 2033 TI - Second-harmonic microscopy of unstained living cardiacmyocytes:?measurements of sarcomere length with 20-nmaccuracy PB - OSA VL - 29 SP - 2031 KW - sarcomere KW - shg AU - Boulesteix, Thierry AU - Beaurepaire, Emmanuel AU - Sauviat, Martin-Pierre AU - Schanne-Klein, Marie-Claire PY - 2004/09/01/ UR - http://dx.doi.org/10.1364/OL.29.002031 ER -