The AcrAB-TolC efflux system of Salmonella enterica serovar Typhimurium plays a role in pathogenesis. Export

@article{Buckley2006, abstract = {The ability of an isogenic set of mutants of Salmonella enterica serovar Typhimurium L354 (SL1344) with defined deletions in genes encoding components of tripartite efflux pumps, including acrB, acrD, acrF and tolC, to colonize chickens was determined in competition with L354. In addition, the ability of L354 and each mutant to adhere to, and invade, human embryonic intestine cells and mouse monocyte macrophages was determined in vitro. The tolC and acrB knockout mutants were hyper-susceptible to a range of antibiotics, dyes and detergents; the tolC mutant was also more susceptible to acid pH and bile and grew more slowly than L354. Complementation of either gene ablated the phenotype. The tolC mutant poorly adhered to both cell types in vitro and was unable to invade macrophages. The acrB mutant adhered, but did not invade macrophages. In vivo, both the acrB mutant and the tolC mutant colonized poorly and did not persist in the avian gut, whereas the acrD and acrF mutant colonized and persisted as well as L354. These data indicate that the AcrAB-TolC system is important for the colonization of chickens by S. Typhimurium and that this system has a role in mediating adherence and uptake into target host cells.}, author = {Buckley, Anthony M. and Webber, Mark A. and Cooles, Sue and Randall, Luke P. and La Ragione, Roberto M. and Woodward, Martin J. and Piddock, Laura J. V.}, citeulike-article-id = {2318657}, citeulike-linkout-0 = {http://dx.doi.org/10.1111/j.1462-5822.2005.00671.x}, doi = {10.1111/j.1462-5822.2005.00671.x}, journal = {Cell Microbiol}, keywords = {adhesion, agents, animal, animals, anti-infective, bacterial, bird, carrier, cell, chickens, diseases, electron, from\_jabref, gastrointestinal, humans, infections, line, macrophages, mice, microbial, microscopy, mutation, proteins, salmonella, sensitivity, tests, tract, typhimurium}, month = {May}, number = {5}, pages = {847--856}, pdf = {Laura\_TolC.pdf}, posted-at = {2008-02-01 09:55:04}, priority = {2}, title = {The AcrAB-TolC efflux system of Salmonella enterica serovar Typhimurium plays a role in pathogenesis.}, url = {10.1111/j.1462-5822.2005.00671.x}, volume = {8}, year = {2006} }

TY - JOUR ID - Buckley2006 L3 - citeulike-article-id:2318657 N2 - The ability of an isogenic set of mutants of Salmonella enterica serovar Typhimurium L354 (SL1344) with defined deletions in genes encoding components of tripartite efflux pumps, including acrB, acrD, acrF and tolC, to colonize chickens was determined in competition with L354. In addition, the ability of L354 and each mutant to adhere to, and invade, human embryonic intestine cells and mouse monocyte macrophages was determined in vitro. The tolC and acrB knockout mutants were hyper-susceptible to a range of antibiotics, dyes and detergents; the tolC mutant was also more susceptible to acid pH and bile and grew more slowly than L354. Complementation of either gene ablated the phenotype. The tolC mutant poorly adhered to both cell types in vitro and was unable to invade macrophages. The acrB mutant adhered, but did not invade macrophages. In vivo, both the acrB mutant and the tolC mutant colonized poorly and did not persist in the avian gut, whereas the acrD and acrF mutant colonized and persisted as well as L354. These data indicate that the AcrAB-TolC system is important for the colonization of chickens by S. Typhimurium and that this system has a role in mediating adherence and uptake into target host cells. IS - 5 JF - Cell Microbiol EP - 856 TI - The AcrAB-TolC efflux system of Salmonella enterica serovar Typhimurium plays a role in pathogenesis. VL - 8 SP - 847 KW - adhesion KW - agents KW - animal KW - animals KW - anti-infective KW - bacterial KW - bird KW - carrier KW - cell KW - chickens KW - diseases KW - electron KW - from_jabref KW - gastrointestinal KW - humans KW - infections KW - line KW - macrophages KW - mice KW - microbial KW - microscopy KW - mutation KW - proteins KW - salmonella KW - sensitivity KW - tests KW - tract KW - typhimurium AU - Buckley, Anthony AU - Webber, Mark AU - Cooles, Sue AU - Randall, Luke AU - La Ragione, Roberto AU - Woodward, Martin AU - Piddock, Laura PY - 2006/May// UR - http://dx.doi.org/10.1111/j.1462-5822.2005.00671.x ER -