Posttranscriptional derepression of GADD45alpha by genotoxic stress. Export

@article{Lal2006, abstract = {The growth arrest- and DNA damage-inducible gene GADD45alpha is potently upregulated in response to stress stimuli. Here, two RNA binding proteins, the mRNA decay-promoting AUF1 and the translational suppressor TIAR, were found to interact specifically with the 3' untranslated region (UTR) of the GADD45alpha mRNA in HeLa cells. These associations were prominent in unstimulated cells, decreasing dramatically after treatment with the genotoxin methyl methanesulfonate (MMS). Analysis of both endogenous and chimeric GADD45alpha mRNA revealed that in untreated cells AUF1 strongly reduced GADD45alpha mRNA stability, whereas TIAR potently inhibited GADD45alpha translation. After genotoxic stress, AUF1 and TIAR dissociated from the GADD45alpha mRNA, thereby allowing coordinated elevations in both GADD45alpha mRNA half-life and translation rate, respectively. We propose that the posttranscriptional derepression of GADD45alpha critically contributes to its potent upregulation after DNA damage.}, author = {Lal, Ashish and Abdelmohsen, Kotb and Pullmann, Rudolf and Kawai, Tomoko and Galban, Stefanie and Yang, Xiaoling and Brewer, Gary and Gorospe, Myriam}, citeulike-article-id = {2318672}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.molcel.2006.03.016}, doi = {10.1016/j.molcel.2006.03.016}, journal = {Mol Cell}, keywords = {3, biosynthesis, cell, cells, cycle, d, damage, dna, end, from\_jabref, half-life, hela, heterogeneous-nuclear, humans, methanesulfonate, methyl, mutagens, nuclear, processing, protein, proteins, regions, ribonucleoprotein, rna, rna-binding, stability, untranslated}, month = {Apr}, number = {1}, pages = {117--128}, pdf = {Lal2006.pdf}, posted-at = {2008-02-01 09:55:06}, priority = {2}, title = {Posttranscriptional derepression of GADD45alpha by genotoxic stress.}, url = {10.1016/j.molcel.2006.03.016}, volume = {22}, year = {2006} }

TY - JOUR ID - Lal2006 L3 - citeulike-article-id:2318672 N2 - The growth arrest- and DNA damage-inducible gene GADD45alpha is potently upregulated in response to stress stimuli. Here, two RNA binding proteins, the mRNA decay-promoting AUF1 and the translational suppressor TIAR, were found to interact specifically with the 3' untranslated region (UTR) of the GADD45alpha mRNA in HeLa cells. These associations were prominent in unstimulated cells, decreasing dramatically after treatment with the genotoxin methyl methanesulfonate (MMS). Analysis of both endogenous and chimeric GADD45alpha mRNA revealed that in untreated cells AUF1 strongly reduced GADD45alpha mRNA stability, whereas TIAR potently inhibited GADD45alpha translation. After genotoxic stress, AUF1 and TIAR dissociated from the GADD45alpha mRNA, thereby allowing coordinated elevations in both GADD45alpha mRNA half-life and translation rate, respectively. We propose that the posttranscriptional derepression of GADD45alpha critically contributes to its potent upregulation after DNA damage. IS - 1 JF - Mol Cell EP - 128 TI - Posttranscriptional derepression of GADD45alpha by genotoxic stress. VL - 22 SP - 117 KW - 3 KW - biosynthesis KW - cell KW - cells KW - cycle KW - d KW - damage KW - dna KW - end KW - from_jabref KW - half-life KW - hela KW - heterogeneous-nuclear KW - humans KW - methanesulfonate KW - methyl KW - mutagens KW - nuclear KW - processing KW - protein KW - proteins KW - regions KW - ribonucleoprotein KW - rna KW - rna-binding KW - stability KW - untranslated AU - Lal, Ashish AU - Abdelmohsen, Kotb AU - Pullmann, Rudolf AU - Kawai, Tomoko AU - Galban, Stefanie AU - Yang, Xiaoling AU - Brewer, Gary AU - Gorospe, Myriam PY - 2006/Apr// UR - http://dx.doi.org/10.1016/j.molcel.2006.03.016 ER -