HY5, Circadian Clock-Associated 1, and a cis-Element, DET1 Dark Response Element, Mediate DET1 Regulation of Chlorophyll a/b-Binding Protein 2 Expression Export

@article{citeulike:3072477, abstract = {DET1 is a pleiotropic regulator of Arabidopsis development and controls the expression of many light-regulated genes. To gain a better understanding of the mechanism by which DET1 controls transcription from light-regulated promoters, we identified elements in the chlorophyll a/b-binding protein 2 (CAB2) promoter that are required for DET1-mediated expression. Using a series of reporter constructs in which the luciferase gene is controlled by CAB2 promoter fragments, we defined two DET1-responsive elements in the CAB2 promoter that are essential for proper CAB2 transcription. A 40-bp DET1 dark-response element (DtRE) is required for both dark and root-specific repression of CAB2, whereas the known CAB upstream factor-1 element is required for DET1 activation-associated effects in the light and repression in the roots. HY5, a factor that binds CAB upstream factor-1, is also required for DET1 effects in the light. DtRE binds two distinct activities in Arabidopsis seedling extracts: a novel activity with binding site CAAAACGC that we have named CAB2 DET1-associated factor 1 plus an activity that is likely to be the myb transcription factor Circadian Clock-Associated 1. Both activities are altered in dark-grown det1 extracts as compared with wild type, correlating a change in extractable DNA binding activity with a major change in CAB2 expression. We conclude that DET1 represses the CAB2 promoter in the dark by regulating the binding of two factors, CAB2 DET1-associated factor 1 and Circadian Clock-Associated 1, to the DtRE. 10.1104/pp.103.025114}, author = {Maxwell, Bridey B. and Andersson, Carol R. and Poole, Daniel S. and Kay, Steve A. and Chory, Joanne}, citeulike-article-id = {3072477}, citeulike-linkout-0 = {http://dx.doi.org/10.1104/pp.103.025114}, citeulike-linkout-1 = {http://www.plantphysiol.org/cgi/content/abstract/133/4/1565}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/14563928}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=14563928}, day = {1}, doi = {10.1104/pp.103.025114}, journal = {Plant Physiol.}, keywords = {arabidopsis, cab2}, month = {December}, number = {4}, pages = {1565--1577}, posted-at = {2008-08-01 15:48:33}, priority = {0}, title = {HY5, Circadian Clock-Associated 1, and a cis-Element, DET1 Dark Response Element, Mediate DET1 Regulation of Chlorophyll a/b-Binding Protein 2 Expression}, url = {http://dx.doi.org/10.1104/pp.103.025114}, volume = {133}, year = {2003} }

TY - JOUR ID - citeulike:3072477 L3 - citeulike-article-id:3072477 N2 - DET1 is a pleiotropic regulator of Arabidopsis development and controls the expression of many light-regulated genes. To gain a better understanding of the mechanism by which DET1 controls transcription from light-regulated promoters, we identified elements in the chlorophyll a/b-binding protein 2 (CAB2) promoter that are required for DET1-mediated expression. Using a series of reporter constructs in which the luciferase gene is controlled by CAB2 promoter fragments, we defined two DET1-responsive elements in the CAB2 promoter that are essential for proper CAB2 transcription. A 40-bp DET1 dark-response element (DtRE) is required for both dark and root-specific repression of CAB2, whereas the known CAB upstream factor-1 element is required for DET1 activation-associated effects in the light and repression in the roots. HY5, a factor that binds CAB upstream factor-1, is also required for DET1 effects in the light. DtRE binds two distinct activities in Arabidopsis seedling extracts: a novel activity with binding site CAAAACGC that we have named CAB2 DET1-associated factor 1 plus an activity that is likely to be the myb transcription factor Circadian Clock-Associated 1. Both activities are altered in dark-grown det1 extracts as compared with wild type, correlating a change in extractable DNA binding activity with a major change in CAB2 expression. We conclude that DET1 represses the CAB2 promoter in the dark by regulating the binding of two factors, CAB2 DET1-associated factor 1 and Circadian Clock-Associated 1, to the DtRE. 10.1104/pp.103.025114 IS - 4 JF - Plant Physiol. EP - 1577 TI - HY5, Circadian Clock-Associated 1, and a cis-Element, DET1 Dark Response Element, Mediate DET1 Regulation of Chlorophyll a/b-Binding Protein 2 Expression VL - 133 SP - 1565 KW - arabidopsis KW - cab2 AU - Maxwell, Bridey AU - Andersson, Carol AU - Poole, Daniel AU - Kay, Steve AU - Chory, Joanne PY - 2003/12/01/ UR - http://dx.doi.org/10.1104/pp.103.025114 ER -