Quantitative Analysis of Drosophila period Gene Transcription in Living Animals

@article{citeulike:3400534, abstract = {To determine the in vivo regulatory pattern of the clock gene period (per), the authors recently developed transgenic Drosophila carrying a luciferase cDNA fused to the promoter region of per. They have now carried out noninvasive, high time-resolution experiments allowing high-throughput monitoring of circadian bioluminescence rhythms in individual living adults for several days. This immediately solved several problems (resulting directly from individual asyn chrony within a population) that have accompanied previous biochemical ex periments in which groups of animals were sacrificed at each time point. Furthermore, the authors have developed numerical analysis methods for auto matically determining rhythmicity associated with bioluminescence records from single flies. This has revealed some features of per gene transcription that were previously unappreciated and provides a general strategy for the analysis of rhythmic time series in the study of molecular rhythms. 10.1177/074873049701200302}, author = {Plautz, Jeffrey D. and Straume, Martin and Stanewsky, Ralf and Jamison, Creston F. and Brandes, Christian and Dowse, Harold B. and Hall, Jeffrey C. and Kay, Steve A.}, citeulike-article-id = {3400534}, journal = {J Biol Rhythms}, keywords = {arabidopsis, circadian\_clock, data\_representation}, month = {June}, number = {3}, pages = {204--217}, posted-at = {2008-10-13 15:59:17}, priority = {4}, title = {Quantitative Analysis of Drosophila period Gene Transcription in Living Animals}, url = {http://jbr.sagepub.com/cgi/content/abstract/12/3/204}, volume = {12}, year = {1997} }

TY - JOUR ID - citeulike:3400534 L3 - citeulike-article-id:3400534 N2 - To determine the in vivo regulatory pattern of the clock gene period (per), the authors recently developed transgenic Drosophila carrying a luciferase cDNA fused to the promoter region of per. They have now carried out noninvasive, high time-resolution experiments allowing high-throughput monitoring of circadian bioluminescence rhythms in individual living adults for several days. This immediately solved several problems (resulting directly from individual asyn chrony within a population) that have accompanied previous biochemical ex periments in which groups of animals were sacrificed at each time point. Furthermore, the authors have developed numerical analysis methods for auto matically determining rhythmicity associated with bioluminescence records from single flies. This has revealed some features of per gene transcription that were previously unappreciated and provides a general strategy for the analysis of rhythmic time series in the study of molecular rhythms. 10.1177/074873049701200302 IS - 3 JF - J Biol Rhythms EP - 217 TI - Quantitative Analysis of Drosophila period Gene Transcription in Living Animals VL - 12 SP - 204 KW - arabidopsis KW - circadian_clock KW - data_representation AU - Plautz, Jeffrey AU - Straume, Martin AU - Stanewsky, Ralf AU - Jamison, Creston AU - Brandes, Christian AU - Dowse, Harold AU - Hall, Jeffrey AU - Kay, Steve PY - 1997/06/01/ UR - http://jbr.sagepub.com/cgi/content/abstract/12/3/204 ER -