Diffuse large B-cell lymphoma: reduced CD20 expression is associated with an inferior survival Export

@article{citeulike:4531674, abstract = {CD19 and CD20 are B cell-specific antigens whose expression is heterogeneous when analyzed by flow cytometry (FCM). We determined the association between CD20 expression and clinical outcome in patients with diffuse large B-cell lymphoma (DLBCL). The mean fluorescence intensity of CD20 and CD19 was determined by FCM, and the cytoplasmic expression of CD20 was determined by immunohistochemistry (IHC) on 272 diagnostic DLBCL samples. Exon 5 of the MS4A1 gene coding for the extracellular component of the CD20 antigen was sequenced in 15 samples. A total of 43 of 272 (16\%) samples had reduced CD20 expression by FCM; of these, 35 (13\%) had bright CD19 expression. The latter had a markedly inferior survival when treated with cyclophosphamide, doxorubicin, vincristine, and prednisone (CHOP) or rituximab-CHOP (R-CHOP; median survival of 1.2 and 3.0 years vs not reached for the others, P < .001 and P = .001), independent of the International Prognostic Index. A total of 41 of 43 samples with reduced CD20 expression by FCM had strong staining for CD20 by IHC. There were no mutations in exon 5 of the MS4A1 gene to explain the discrepancy between FCM and IHC. CD20 and CD19 expression by FCM should be determined on all biopsies of patients with DLBCL because reduced CD20 expression cannot be reliably detected by IHC. 10.1182/blood-2008-09-177469}, author = {Johnson, Nathalie A. and Boyle, Merrill and Bashashati, Ali and Leach, Stephen and Brooks-Wilson, Angela and Sehn, Laurie H. and Chhanabhai, Mukesh and Brinkman, Ryan R. and Connors, Joseph M. and Weng, Andrew P. and Gascoyne, Randy D.}, citeulike-article-id = {4531674}, citeulike-linkout-0 = {http://dx.doi.org/10.1182/blood-2008-09-177469}, citeulike-linkout-1 = {http://bloodjournal.hematologylibrary.org/cgi/content/abstract/113/16/3773}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19029441}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19029441}, day = {16}, doi = {10.1182/blood-2008-09-177469}, journal = {Blood}, keywords = {lymphoma, nhl}, month = {April}, number = {16}, pages = {3773--3780}, posted-at = {2009-05-17 15:04:31}, priority = {2}, title = {Diffuse large B-cell lymphoma: reduced CD20 expression is associated with an inferior survival}, url = {http://dx.doi.org/10.1182/blood-2008-09-177469}, volume = {113}, year = {2009} }

TY - JOUR ID - citeulike:4531674 L3 - citeulike-article-id:4531674 N2 - CD19 and CD20 are B cell-specific antigens whose expression is heterogeneous when analyzed by flow cytometry (FCM). We determined the association between CD20 expression and clinical outcome in patients with diffuse large B-cell lymphoma (DLBCL). The mean fluorescence intensity of CD20 and CD19 was determined by FCM, and the cytoplasmic expression of CD20 was determined by immunohistochemistry (IHC) on 272 diagnostic DLBCL samples. Exon 5 of the MS4A1 gene coding for the extracellular component of the CD20 antigen was sequenced in 15 samples. A total of 43 of 272 (16%) samples had reduced CD20 expression by FCM; of these, 35 (13%) had bright CD19 expression. The latter had a markedly inferior survival when treated with cyclophosphamide, doxorubicin, vincristine, and prednisone (CHOP) or rituximab-CHOP (R-CHOP; median survival of 1.2 and 3.0 years vs not reached for the others, P < .001 and P = .001), independent of the International Prognostic Index. A total of 41 of 43 samples with reduced CD20 expression by FCM had strong staining for CD20 by IHC. There were no mutations in exon 5 of the MS4A1 gene to explain the discrepancy between FCM and IHC. CD20 and CD19 expression by FCM should be determined on all biopsies of patients with DLBCL because reduced CD20 expression cannot be reliably detected by IHC. 10.1182/blood-2008-09-177469 IS - 16 JF - Blood EP - 3780 TI - Diffuse large B-cell lymphoma: reduced CD20 expression is associated with an inferior survival VL - 113 SP - 3773 KW - lymphoma KW - nhl AU - Johnson, Nathalie AU - Boyle, Merrill AU - Bashashati, Ali AU - Leach, Stephen AU - Brooks-Wilson, Angela AU - Sehn, Laurie AU - Chhanabhai, Mukesh AU - Brinkman, Ryan AU - Connors, Joseph AU - Weng, Andrew AU - Gascoyne, Randy PY - 2009/04/16/ UR - http://dx.doi.org/10.1182/blood-2008-09-177469 ER -