Association of the Cell Cycle Transcription Factor Mbp1 with the Skn7 Response Regulator in Budding Yeast Export

@article{citeulike:1300962, abstract = {We previously isolated the SKN7 gene in a screen designed to isolate new components of the G1-S cell cycle transcription machinery in budding yeast. We have now found that Skn7 associates with Mbp1, the DNA-binding component of the G1-S transcription factor DSC1/MBF. SKN7 and MBP1 show several genetic interactions. Skn7 overexpression is lethal and is suppressed by a mutation in MBP1. Similarly, high overexpression of Mbp1 is lethal and can be suppressed by skn7 mutations. SKN7 is also required for MBP1 function in a mutant compromised for G1-specific transcription. Gel-retardation assays indicate that Skn7 is not an integral part of MBF. However, a physical interaction between Skn7 and Mbp1 was detected using two-hybrid assays and GST pulldowns. Thus, Skn7 and Mbp1 seem to form a transcription factor independent of MBF. Genetic data suggest that this new transcription factor could be involved in the bud-emergence process.}, author = {Bouquin, Nicolas and Johnson, Anthony L. and Morgan, Brian A. and Johnston, Leland H.}, citeulike-article-id = {1300962}, citeulike-linkout-0 = {http://www.molbiolcell.org/cgi/content/abstract/10/10/3389}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/10512874}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=10512874}, day = {1}, journal = {Mol. Biol. Cell}, keywords = {cutoff\_model, skn7}, month = {October}, number = {10}, pages = {3389--3400}, posted-at = {2007-05-16 23:47:44}, priority = {2}, title = {Association of the Cell Cycle Transcription Factor Mbp1 with the Skn7 Response Regulator in Budding Yeast}, url = {http://www.molbiolcell.org/cgi/content/abstract/10/10/3389}, volume = {10}, year = {1999} }

TY - JOUR ID - citeulike:1300962 L3 - citeulike-article-id:1300962 N2 - We previously isolated the SKN7 gene in a screen designed to isolate new components of the G1-S cell cycle transcription machinery in budding yeast. We have now found that Skn7 associates with Mbp1, the DNA-binding component of the G1-S transcription factor DSC1/MBF. SKN7 and MBP1 show several genetic interactions. Skn7 overexpression is lethal and is suppressed by a mutation in MBP1. Similarly, high overexpression of Mbp1 is lethal and can be suppressed by skn7 mutations. SKN7 is also required for MBP1 function in a mutant compromised for G1-specific transcription. Gel-retardation assays indicate that Skn7 is not an integral part of MBF. However, a physical interaction between Skn7 and Mbp1 was detected using two-hybrid assays and GST pulldowns. Thus, Skn7 and Mbp1 seem to form a transcription factor independent of MBF. Genetic data suggest that this new transcription factor could be involved in the bud-emergence process. IS - 10 JF - Mol. Biol. Cell EP - 3400 TI - Association of the Cell Cycle Transcription Factor Mbp1 with the Skn7 Response Regulator in Budding Yeast VL - 10 SP - 3389 KW - cutoff_model KW - skn7 AU - Bouquin, Nicolas AU - Johnson, Anthony AU - Morgan, Brian AU - Johnston, Leland PY - 1999/10/01/ UR - http://www.molbiolcell.org/cgi/content/abstract/10/10/3389 ER -