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macowell's physcomitrella [5 articles]

 
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✔ Cloning and Characterization of Glycine-Rich RNA-Binding Protein cDNAs in the Moss Physcomitrella patens

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Plant and Cell Physiology, Vol. 45, No. 1. (15 January 2004), pp. 48-56, doi:10.1093/pcp/pch005
by Tsuyoshi Nomata, Yukihiro Kabeya, Naoki Sato
posted to genetics physcomitrella teamexample by macowell ✚ on 2007-07-03 02:07:46 ** along with 2 people and 1 group dandaman physcomitrella BUiGEM
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Abstract

We isolated three cDNAs for the genes PpGRP1, PpGRP2 and PpGRP3 that encode glycine-rich RNA-binding proteins (GRPs) from Physcomitrella patens. Three full-length cDNA clones were isolated from a cDNA library prepared from poly(A)+ RNA from 7-day-old protonemata of P. patens. They were named PpGRP1, PpGRP2 and PpGRP3, which encode putative polypeptides of 162, 178 and 155 residues, respectively. Preliminary genomic sequencing suggested that the positions of the three introns in the PpGRP3 gene are similar to those of introns in Arabidopsis ...
 

✔ Quantitative promoter analysis in Physcomitrella patens: a set of plant vectors activating gene expression within three orders of magnitude.

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BMC Biotechnol, Vol. 4 (7 July 2004), doi:10.1186/1472-6750-4-13
by V. Horstmann, C. M. Huether, W. Jost, R. Reski, E. L. Decker
posted to physcomitrella teamexample by macowell ✚ on 2007-07-03 02:04:58 ** along with 1 person and 1 group physcomitrella BUiGEM
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BACKGROUND: In addition to studies of plant gene function and developmental analyses, plant biotechnological use is largely dependent upon transgenic technologies. The moss Physcomitrella patens has become an exciting model system for studying plant molecular processes due to an exceptionally high rate of nuclear gene targeting by homologous recombination compared with other plants. However, its use in transgenic approaches requires expression vectors that incorporate sufficiently strong promoters. To satisfy this requirement, a set of plant expression vectors was constructed and equipped ...
 

DEVELOPMENT, GENETICS AND MOLECULAR BIOLOGY OF MOSSES

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Botanica acta, Vol. 111, No. 11. (1998), pp. 1-15
by R. Reski
posted to physcomitrella teamexample by macowell ✚ on 2007-07-03 01:52:38 ** along with 1 group BUiGEM
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✔ Efficient gene targeting in the moss Physcomitrella patens

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The Plant Journal, Vol. 11, No. 6. (June 1997), pp. 1195-1206, doi:10.1046/j.1365-313x.1997.11061195.x
by Didier G. Schaefer, Jean-Pierre Zryd
posted to physcomitrella teamexample by macowell ✚ on 2007-07-03 01:52:00 *** along with 2 people and 1 group natstreet physcomitrella BUiGEM
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The moss Physcomitrella patens is used as a genetic model system to study plant development, taking advantage of the fact that the haploid gametophyte dominates in its life cycle. Transformation experiments designed to target three single-copy genomic loci were performed to determine the efficiency of gene targeting in this plant. Mean transformation rates were 10-fold higher with the targeting vectors and molecular evidence for the integration of exogenous DNA into each targeted locus by homologous recombination is provided. The efficiency of ...
 

✔ Mosses as model systems

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Trends in Plant Science, Vol. 2, No. 3. (March 1997), pp. 99-105
by David J. Cove, Celia D. Knight, Tilman Lamparter
posted to physcomitrella teamexample by macowell ✚ on 2007-07-03 01:44:12 **** along with 1 person and 1 group physcomitrella BUiGEM
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Abstract

Mosses hold many attractions as model organisms for research in plant science. Their position as the simplest of land plants makes them central to the study of plant evolution, particularly in shedding light on how their aquatic predecessors evolved to survive on land. The use of mosses for developmental studies hinges on the ability to observe development in living material at the level of the individual cell. However, more recently techniques for the molecular analysis of mosses have provided tools for ...
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