The MspA porin promotes growth and increases antibiotic susceptibility of both Mycobacterium bovis BCG and Mycobacterium tuberculosis. Export

@article{citeulike:771162, abstract = {Porins mediate the diffusion of hydrophilic solutes across the outer membrane of mycobacteria, but the efficiency of this pathway is very low compared to Gram-negative bacteria. To examine the importance of porins in slow-growing mycobacteria, the major porin MspA of Mycobacterium smegmatis was expressed in Mycobacterium tuberculosis and Mycobacterium bovis. Approximately 20 and 35 MspA molecules per microm(2) cell wall were observed in M. tuberculosis and M. bovis BCG, respectively, by electron microscopy and quantitative immunoblot experiments. Surface accessibility of MspA in M. tuberculosis was demonstrated by flow cytometry. Glucose uptake was twofold faster, indicating that the outer membrane permeability of M. bovis BCG to small and hydrophilic solutes was increased by MspA. This significantly accelerated the growth of M. bovis BCG, identifying very slow nutrient uptake as one of the determinants of slow growth in mycobacteria. The susceptibility of both M. bovis BCG and M. tuberculosis to zwitterionic beta-lactam antibiotics was substantially enhanced by MspA, decreasing the minimal inhibitory concentration up to 16-fold. Furthermore, M. tuberculosis became significantly more susceptible to isoniazid, ethambutol and streptomycin. Fluorescence with the nucleic acid binding dye SYTO 9 was 10-fold increased upon expression of mspA. These results indicated that MspA not only enhanced the efficiency of the porin pathway, but also that of pathways mediating access to large and/or hydrophobic agents. This study provides the first experimental evidence that porins are important for drug susceptibility of M. tuberculosis.}, address = {Lehrstuhl f\"{u}r Mikrobiologie, Friedrich-Alexander-Universit\"{a}t Erlangen-N\"{u}rnberg, Staudtstr 5, D-91058 Erlangen, Germany.}, author = {Mailaender, C. and Reiling, N. and Engelhardt, H. and Bossmann, S. and Ehlers, S. and Niederweis, M.}, citeulike-article-id = {771162}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/15073295}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=15073295}, issn = {1350-0872}, journal = {Microbiology}, keywords = {bacterial\_growth, bcg, mtuberculosis, porins}, month = {April}, number = {Pt 4}, pages = {853--864}, posted-at = {2006-07-24 14:52:29}, priority = {2}, title = {The MspA porin promotes growth and increases antibiotic susceptibility of both Mycobacterium bovis BCG and Mycobacterium tuberculosis.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/15073295}, volume = {150}, year = {2004} }

TY - JOUR ID - citeulike:771162 L3 - citeulike-article-id:771162 N2 - Porins mediate the diffusion of hydrophilic solutes across the outer membrane of mycobacteria, but the efficiency of this pathway is very low compared to Gram-negative bacteria. To examine the importance of porins in slow-growing mycobacteria, the major porin MspA of Mycobacterium smegmatis was expressed in Mycobacterium tuberculosis and Mycobacterium bovis. Approximately 20 and 35 MspA molecules per microm(2) cell wall were observed in M. tuberculosis and M. bovis BCG, respectively, by electron microscopy and quantitative immunoblot experiments. Surface accessibility of MspA in M. tuberculosis was demonstrated by flow cytometry. Glucose uptake was twofold faster, indicating that the outer membrane permeability of M. bovis BCG to small and hydrophilic solutes was increased by MspA. This significantly accelerated the growth of M. bovis BCG, identifying very slow nutrient uptake as one of the determinants of slow growth in mycobacteria. The susceptibility of both M. bovis BCG and M. tuberculosis to zwitterionic beta-lactam antibiotics was substantially enhanced by MspA, decreasing the minimal inhibitory concentration up to 16-fold. Furthermore, M. tuberculosis became significantly more susceptible to isoniazid, ethambutol and streptomycin. Fluorescence with the nucleic acid binding dye SYTO 9 was 10-fold increased upon expression of mspA. These results indicated that MspA not only enhanced the efficiency of the porin pathway, but also that of pathways mediating access to large and/or hydrophobic agents. This study provides the first experimental evidence that porins are important for drug susceptibility of M. tuberculosis. IS - Pt 4 JF - Microbiology SN - 1350-0872 AD - Lehrstuhl für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr 5, D-91058 Erlangen, Germany. EP - 864 TI - The MspA porin promotes growth and increases antibiotic susceptibility of both Mycobacterium bovis BCG and Mycobacterium tuberculosis. VL - 150 SP - 853 KW - bacterial_growth KW - bcg KW - mtuberculosis KW - porins AU - Mailaender, C AU - Reiling, N AU - Engelhardt, H AU - Bossmann, S AU - Ehlers, S AU - Niederweis, M PY - 2004/04// UR - http://view.ncbi.nlm.nih.gov/pubmed/15073295 ER -