Independent recruitment in vivo by Gal4 of two complexes required for transcription.

by: GO Bryant, M Ptashne

Molecular cell, Vol. 11, No. 5. (May 2003), pp. 1301-1309.

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Abstract

We use a modified form of ChIP to analyze the recruitment of seven sets of proteins to the yeast GAL genes upon induction. We resolve three stages of recruitment: first SAGA, then Mediator, and finally Pol II along with four other proteins (including TBP) bind the promoter. In a strain lacking SAGA, Mediator is recruited with a time course indistinguishable from that observed in wild-type cells. Our results are consistent with the notion that a single species of activator, Gal4, separately contacts, and thereby directly recruits, SAGA and Mediator.

@article{citeulike:3021155, abstract = {We use a modified form of ChIP to analyze the recruitment of seven sets of proteins to the yeast GAL genes upon induction. We resolve three stages of recruitment: first SAGA, then Mediator, and finally Pol II along with four other proteins (including TBP) bind the promoter. In a strain lacking SAGA, Mediator is recruited with a time course indistinguishable from that observed in wild-type cells. Our results are consistent with the notion that a single species of activator, Gal4, separately contacts, and thereby directly recruits, SAGA and Mediator.}, address = {Molecular Biology Program, Sloan-Kettering Institute, New York, NY 10021, USA.}, author = {Bryant, G. O. and Ptashne, M. }, citeulike-article-id = {3021155}, issn = {1097-2765}, journal = {Molecular cell}, keywords = {chip, gal4}, month = {May}, number = {5}, pages = {1301--1309}, posted-at = {2008-07-19 18:59:02}, priority = {2}, title = {Independent recruitment in vivo by Gal4 of two complexes required for transcription.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/12769853}, volume = {11}, year = {2003} }

RIS record

TY - JOUR ID - citeulike:3021155 L3 - citeulike-article-id:3021155 TI - Independent recruitment in vivo by Gal4 of two complexes required for transcription. JF - Molecular cell VL - 11 IS - 5 SP - 1301 EP - 1309 AD - Molecular Biology Program, Sloan-Kettering Institute, New York, NY 10021, USA. SN - 1097-2765 N2 - We use a modified form of ChIP to analyze the recruitment of seven sets of proteins to the yeast GAL genes upon induction. We resolve three stages of recruitment: first SAGA, then Mediator, and finally Pol II along with four other proteins (including TBP) bind the promoter. In a strain lacking SAGA, Mediator is recruited with a time course indistinguishable from that observed in wild-type cells. Our results are consistent with the notion that a single species of activator, Gal4, separately contacts, and thereby directly recruits, SAGA and Mediator. KW - chip KW - gal4 AU - Bryant, GO AU - Ptashne, M PY - 2003/05// UR - http://view.ncbi.nlm.nih.gov/pubmed/12769853 ER -

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