The yeast SWI-SNF complex facilitates binding of a transcriptional activator to nucleosomal sites in vivo. Export

@article{citeulike:3089687, abstract = {The Saccharomyces cerevisiae SWI-SNF complex is a 2-MDa protein assembly that is required for the function of many transcriptional activators. Here we describe experiments on the role of the SWI-SNF complex in activation of transcription by the yeast activator GAL4. We find that while SWI-SNF activity is not required for the GAL4 activator to bind to and activate transcription from nucleosome-free binding sites, the complex is required for GAL4 to bind to and function at low-affinity, nucleosomal binding sites in vivo. This SWI-SNF dependence can be overcome by (i) replacing the low-affinity sites with higher-affinity, consensus GAL4 binding sequences or (ii) placing the low-affinity sites into a nucleosome-free region. These results define the criteria for the SWI-SNF dependence of gene expression and provide the first in vivo evidence that the SWI-SNF complex can regulate gene expression by modulating the DNA binding of an upstream activator protein.}, address = {Department of Biochemistry and Molecular Biology, University of Massachusetts Medical Center, Worcester 01605, USA.}, author = {Burns, L. G. and Peterson, C. L.}, citeulike-article-id = {3089687}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/9234737}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=9234737}, issn = {0270-7306}, journal = {Molecular and cellular biology}, keywords = {activator, binding, chromatin, dna, gal4}, month = {August}, number = {8}, pages = {4811--4819}, posted-at = {2008-08-05 22:39:01}, priority = {3}, title = {The yeast SWI-SNF complex facilitates binding of a transcriptional activator to nucleosomal sites in vivo.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9234737}, volume = {17}, year = {1997} }

TY - JOUR ID - citeulike:3089687 L3 - citeulike-article-id:3089687 N2 - The Saccharomyces cerevisiae SWI-SNF complex is a 2-MDa protein assembly that is required for the function of many transcriptional activators. Here we describe experiments on the role of the SWI-SNF complex in activation of transcription by the yeast activator GAL4. We find that while SWI-SNF activity is not required for the GAL4 activator to bind to and activate transcription from nucleosome-free binding sites, the complex is required for GAL4 to bind to and function at low-affinity, nucleosomal binding sites in vivo. This SWI-SNF dependence can be overcome by (i) replacing the low-affinity sites with higher-affinity, consensus GAL4 binding sequences or (ii) placing the low-affinity sites into a nucleosome-free region. These results define the criteria for the SWI-SNF dependence of gene expression and provide the first in vivo evidence that the SWI-SNF complex can regulate gene expression by modulating the DNA binding of an upstream activator protein. IS - 8 JF - Molecular and cellular biology SN - 0270-7306 AD - Department of Biochemistry and Molecular Biology, University of Massachusetts Medical Center, Worcester 01605, USA. EP - 4819 TI - The yeast SWI-SNF complex facilitates binding of a transcriptional activator to nucleosomal sites in vivo. VL - 17 SP - 4811 KW - activator KW - binding KW - chromatin KW - dna KW - gal4 AU - Burns, LG AU - Peterson, CL PY - 1997/08// UR - http://view.ncbi.nlm.nih.gov/pubmed/9234737 ER -