A eukaryotic transcriptional activator bearing the DNA specificity of a prokaryotic repressor Export

@article{citeulike:3158571, abstract = {We describe a new protein that binds to DNA and activates gene transcription in yeast. This protein, LexAGAL4, is a hybrid of LexA, an Escherichia coli repressor protein, and GAL4, a Saccharomyces cerevisiae transcriptional activator. The hybrid protein, synthesized in yeast, activates transcription of a gene if and only if a lexA operator is present near the transcription start site. Thus, the DNA binding function of GAL4 can be replaced with that of a prokaryotic repressor without loss of the transcriptional activation function. These results suggest that DNA-bound LexA-GAL4 and DNA-bound GAL4 activate transcription by contacting other proteins.}, author = {Brent, Roger and Ptashne, Mark}, citeulike-article-id = {3158571}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/0092-8674(85)90246-6}, citeulike-linkout-1 = {http://www.sciencedirect.com/science/article/B6WSN-4C89DC2-S/2/c8e621ae60d0dc096d9f7ded35ef20be}, doi = {10.1016/0092-8674(85)90246-6}, journal = {Cell}, keywords = {activation, domain-swapping, gal4, lexa, mapplab-classic, repressor, transcription}, month = {December}, number = {3, Part 2}, pages = {729--736}, posted-at = {2008-08-27 16:50:51}, priority = {2}, title = {A eukaryotic transcriptional activator bearing the DNA specificity of a prokaryotic repressor}, url = {http://dx.doi.org/10.1016/0092-8674(85)90246-6}, volume = {43}, year = {1985} }

TY - JOUR ID - citeulike:3158571 L3 - citeulike-article-id:3158571 N2 - We describe a new protein that binds to DNA and activates gene transcription in yeast. This protein, LexAGAL4, is a hybrid of LexA, an Escherichia coli repressor protein, and GAL4, a Saccharomyces cerevisiae transcriptional activator. The hybrid protein, synthesized in yeast, activates transcription of a gene if and only if a lexA operator is present near the transcription start site. Thus, the DNA binding function of GAL4 can be replaced with that of a prokaryotic repressor without loss of the transcriptional activation function. These results suggest that DNA-bound LexA-GAL4 and DNA-bound GAL4 activate transcription by contacting other proteins. IS - 3, Part 2 JF - Cell EP - 736 TI - A eukaryotic transcriptional activator bearing the DNA specificity of a prokaryotic repressor VL - 43 SP - 729 KW - activation KW - domain-swapping KW - gal4 KW - lexa KW - mapplab-classic KW - repressor KW - transcription AU - Brent, Roger AU - Ptashne, Mark PY - 1985/12// UR - http://dx.doi.org/10.1016/0092-8674(85)90246-6 ER -