Activated transcription via mammalian amino acid response elements does not require enhanced recruitment of the Mediator complex Export

@article{citeulike:3371529, abstract = {It is unclear whether Mediator complex in yeast is necessary for all RNA polymerase II (Pol II) transcription or if it is limited to genes activated by environmental stress. In mammals, amino acid limitation induces SNAT2 transcription through ATF4 binding at an amino acid response element. ATF4 is the functional counterpart to the yeast amino acid-dependent regulator GCN4 and GCN4 recruits Mediator during transcriptional activation. Consistent with enhanced SNAT2 transcription activity, the present data demonstrate that amino acid limitation increased SNAT2 promoter association of the general transcription factors that make up the preinitiation complex, including Pol II, but there was no increase in Mediator recruitment. Furthermore, siRNA knockdown of eight Mediator subunits caused no significant decrease in SNAT2 transcription. The estrogen-dependent pS2 gene was used as a positive control for both the ChIP and the siRNA approaches and the data demonstrated the requirement for Mediator recruitment. These results document that activation of the SNAT2 gene by the mammalian amino acid response pathway occurs independently of enhanced Mediator recruitment. 10.1093/nar/gkn538}, author = {Thiaville, Michelle M. and Dudenhausen, Elizabeth E. and Awad, Keytam S. and Gjymishka, Altin and Zhong, Can and Kilberg, Michael S.}, citeulike-article-id = {3371529}, citeulike-linkout-0 = {http://dx.doi.org/10.1093/nar/gkn538}, citeulike-linkout-1 = {http://nar.oxfordjournals.org/cgi/content/abstract/36/17/5571}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18757893}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18757893}, day = {1}, doi = {10.1093/nar/gkn538}, journal = {Nucl. Acids Res.}, keywords = {journalclub, med1, med15gal11, med23, mediator, sirna}, month = {October}, number = {17}, pages = {5571--5580}, posted-at = {2008-10-03 20:14:12}, priority = {2}, title = {Activated transcription via mammalian amino acid response elements does not require enhanced recruitment of the Mediator complex}, url = {http://dx.doi.org/10.1093/nar/gkn538}, volume = {36}, year = {2008} }

TY - JOUR ID - citeulike:3371529 L3 - citeulike-article-id:3371529 N2 - It is unclear whether Mediator complex in yeast is necessary for all RNA polymerase II (Pol II) transcription or if it is limited to genes activated by environmental stress. In mammals, amino acid limitation induces SNAT2 transcription through ATF4 binding at an amino acid response element. ATF4 is the functional counterpart to the yeast amino acid-dependent regulator GCN4 and GCN4 recruits Mediator during transcriptional activation. Consistent with enhanced SNAT2 transcription activity, the present data demonstrate that amino acid limitation increased SNAT2 promoter association of the general transcription factors that make up the preinitiation complex, including Pol II, but there was no increase in Mediator recruitment. Furthermore, siRNA knockdown of eight Mediator subunits caused no significant decrease in SNAT2 transcription. The estrogen-dependent pS2 gene was used as a positive control for both the ChIP and the siRNA approaches and the data demonstrated the requirement for Mediator recruitment. These results document that activation of the SNAT2 gene by the mammalian amino acid response pathway occurs independently of enhanced Mediator recruitment. 10.1093/nar/gkn538 IS - 17 JF - Nucl. Acids Res. EP - 5580 TI - Activated transcription via mammalian amino acid response elements does not require enhanced recruitment of the Mediator complex VL - 36 SP - 5571 KW - journalclub KW - med1 KW - med15gal11 KW - med23 KW - mediator KW - sirna AU - Thiaville, Michelle AU - Dudenhausen, Elizabeth AU - Awad, Keytam AU - Gjymishka, Altin AU - Zhong, Can AU - Kilberg, Michael PY - 2008/10/01/ UR - http://dx.doi.org/10.1093/nar/gkn538 ER -