RNA-Seq: a revolutionary tool for transcriptomics Export

by: Zhong Wang, Mark Gerstein, Michael Snyder

Nature Reviews Genetics, Vol. 10, No. 1. (01 January 2009), pp. 57-63.

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Abstract

RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. This article describes the RNA-Seq approach, the challenges associated with its application, and the advances made so far in characterizing several eukaryote transcriptomes.

@article{citeulike:3614773, abstract = {RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. This article describes the RNA-Seq approach, the challenges associated with its application, and the advances made so far in characterizing several eukaryote transcriptomes.}, author = {Wang, Zhong and Gerstein, Mark and Snyder, Michael}, citeulike-article-id = {3614773}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nrg2484}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nrg2484}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19015660}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19015660}, day = {01}, doi = {10.1038/nrg2484}, issn = {1471-0056}, journal = {Nature Reviews Genetics}, keywords = {rna\_seq, transcriptome}, month = {January}, number = {1}, pages = {57--63}, posted-at = {2009-11-06 23:20:46}, priority = {2}, publisher = {Nature Publishing Group}, title = {RNA-Seq: a revolutionary tool for transcriptomics}, url = {http://dx.doi.org/10.1038/nrg2484}, volume = {10}, year = {2009} }

RIS record

TY - JOUR ID - citeulike:3614773 L3 - citeulike-article-id:3614773 N2 - RNA-Seq is a recently developed approach to transcriptome profiling that uses deep-sequencing technologies. Studies using this method have already altered our view of the extent and complexity of eukaryotic transcriptomes. RNA-Seq also provides a far more precise measurement of levels of transcripts and their isoforms than other methods. This article describes the RNA-Seq approach, the challenges associated with its application, and the advances made so far in characterizing several eukaryote transcriptomes. IS - 1 JF - Nature Reviews Genetics SN - 1471-0056 EP - 63 TI - RNA-Seq: a revolutionary tool for transcriptomics PB - Nature Publishing Group VL - 10 SP - 57 KW - rna_seq KW - transcriptome AU - Wang, Zhong AU - Gerstein, Mark AU - Snyder, Michael PY - 2009/01/01/ UR - http://dx.doi.org/10.1038/nrg2484 ER -

RNA-Seq: a revolutionary tool for transcriptomics Export

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