Widespread Translational Inhibition by Plant miRNAs and siRNAs Export

@article{citeulike:2804733, abstract = {High complementarity between plant miRNAs and their mRNA targets is thought to cause silencing prevalently by endonucleolytic cleavage. We have isolated Arabidopsis mutants defective in miRNA action. Their analysis provides evidence that plant miRNA-guided silencing has a widespread translational inhibitory component that is genetically separable from endonucleolytic cleavage. We further show that the same is true of silencing mediated by short interfering (si)RNA populations. Translational repression is effected in part by the ARGONAUTE proteins AGO1 and AGO10. It also requires the activity of the microtubule-severing enzyme katanin, implicating cytoskeleton dynamics in miRNA action as recently suggested from animal studies. Also as in animals, the decapping component VCS/Ge-1 is required for translational repression by miRNAs, suggesting that the underlying mechanisms in the two kingdoms are related. 10.1126/science.1159151}, author = {Brodersen, Peter and Sakvarelidze-Achard, Lali and Bruun-Rasmussen, Marianne and Dunoyer, Patrice and Yamamoto, Yoshiharu Y. and Sieburth, Leslie and Voinnet, Olivier}, citeulike-article-id = {2804733}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1159151}, citeulike-linkout-1 = {http://www.sciencemag.org/cgi/content/abstract/1159151//}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18483398}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18483398}, day = {15}, doi = {10.1126/science.1159151}, journal = {Science}, keywords = {transcription}, month = {May}, pages = {1159151+}, posted-at = {2008-05-18 22:42:58}, priority = {2}, title = {Widespread Translational Inhibition by Plant miRNAs and siRNAs}, url = {http://dx.doi.org/10.1126/science.1159151}, year = {2008} }

TY - JOUR ID - citeulike:2804733 L3 - citeulike-article-id:2804733 N2 - High complementarity between plant miRNAs and their mRNA targets is thought to cause silencing prevalently by endonucleolytic cleavage. We have isolated Arabidopsis mutants defective in miRNA action. Their analysis provides evidence that plant miRNA-guided silencing has a widespread translational inhibitory component that is genetically separable from endonucleolytic cleavage. We further show that the same is true of silencing mediated by short interfering (si)RNA populations. Translational repression is effected in part by the ARGONAUTE proteins AGO1 and AGO10. It also requires the activity of the microtubule-severing enzyme katanin, implicating cytoskeleton dynamics in miRNA action as recently suggested from animal studies. Also as in animals, the decapping component VCS/Ge-1 is required for translational repression by miRNAs, suggesting that the underlying mechanisms in the two kingdoms are related. 10.1126/science.1159151 JF - Science TI - Widespread Translational Inhibition by Plant miRNAs and siRNAs SP - 1159151 KW - transcription AU - Brodersen, Peter AU - Sakvarelidze-Achard, Lali AU - Bruun-Rasmussen, Marianne AU - Dunoyer, Patrice AU - Yamamoto, Yoshiharu AU - Sieburth, Leslie AU - Voinnet, Olivier PY - 2008/05/15/ UR - http://dx.doi.org/10.1126/science.1159151 ER -