Genetically encoded short peptide tag for versatile protein labeling by Sfp phosphopantetheinyl transferase Export

@article{citeulike:1923264, abstract = {10.1073/pnas.0507705102 An 11-residue peptide with the sequence DLEFIASKLA was identified from a genomic library of by phage display as an efficient substrate for Sfp phosphopantetheinyl transferase-catalyzed protein labeling by small molecule-CoA conjugates. We name this peptide the \^{a}€œybbR tag,\^{a}€ because part of its sequence is derived from the ORF in the genome. The site of Sfp-catalyzed ybbR tag labeling was mapped to the underlined Ser residue, and the ybbR tag was found to have a strong tendency for adopting an \^{I}±-helical conformation in solution. Here we demonstrate that the ybbR tag can be fused to the N or C termini of target proteins or inserted in a flexible loop in the middle of a target protein for site-specific protein labeling by Sfp. The short size of the ybbR tag and its compatibility with various target proteins, the broad substrate specificity of Sfp for labeling the ybbR tag with small-molecule probes of diverse structures, and the high specificity and efficiency of the labeling reaction make Sfp-catalyzed ybbR tag labeling an attractive tool for expanding protein structural and functional diversities by posttranslational modification.}, address = {Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.}, author = {Yin, Jun and Straight, Paul D. and McLoughlin, Shaun M. and Zhou, Zhe and Lin, Alison J. and Golan, David E. and Kelleher, Neil L. and Kolter, Roberto and Walsh, Christopher T.}, citeulike-article-id = {1923264}, citeulike-linkout-0 = {http://dx.doi.org/10.1073/pnas.0507705102}, citeulike-linkout-1 = {http://www.pnas.org/content/102/44/15815.abstract}, citeulike-linkout-2 = {http://www.pnas.org/content/102/44/15815.full.pdf}, citeulike-linkout-3 = {http://view.ncbi.nlm.nih.gov/pubmed/16236721}, citeulike-linkout-4 = {http://www.hubmed.org/display.cgi?uids=16236721}, day = {1}, doi = {10.1073/pnas.0507705102}, issn = {0027-8424}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, keywords = {labeling, sfp}, month = {November}, number = {44}, pages = {15815--15820}, posted-at = {2009-10-26 17:08:28}, priority = {2}, title = {Genetically encoded short peptide tag for versatile protein labeling by Sfp phosphopantetheinyl transferase}, url = {http://dx.doi.org/10.1073/pnas.0507705102}, volume = {102}, year = {2005} }

TY - JOUR ID - citeulike:1923264 L3 - citeulike-article-id:1923264 N2 - 10.1073/pnas.0507705102 An 11-residue peptide with the sequence DLEFIASKLA was identified from a genomic library of by phage display as an efficient substrate for Sfp phosphopantetheinyl transferase-catalyzed protein labeling by small molecule-CoA conjugates. We name this peptide the “ybbR tag,” because part of its sequence is derived from the ORF in the genome. The site of Sfp-catalyzed ybbR tag labeling was mapped to the underlined Ser residue, and the ybbR tag was found to have a strong tendency for adopting an α-helical conformation in solution. Here we demonstrate that the ybbR tag can be fused to the N or C termini of target proteins or inserted in a flexible loop in the middle of a target protein for site-specific protein labeling by Sfp. The short size of the ybbR tag and its compatibility with various target proteins, the broad substrate specificity of Sfp for labeling the ybbR tag with small-molecule probes of diverse structures, and the high specificity and efficiency of the labeling reaction make Sfp-catalyzed ybbR tag labeling an attractive tool for expanding protein structural and functional diversities by posttranslational modification. IS - 44 JF - Proceedings of the National Academy of Sciences of the United States of America SN - 0027-8424 AD - Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA. EP - 15820 TI - Genetically encoded short peptide tag for versatile protein labeling by Sfp phosphopantetheinyl transferase VL - 102 SP - 15815 KW - labeling KW - sfp AU - Yin, Jun AU - Straight, Paul AU - McLoughlin, Shaun AU - Zhou, Zhe AU - Lin, Alison AU - Golan, David AU - Kelleher, Neil AU - Kolter, Roberto AU - Walsh, Christopher PY - 2005/11/01/ UR - http://dx.doi.org/10.1073/pnas.0507705102 ER -