The E. coli alpha-hemolysin secretion system and its use in vaccine development. Export

@article{citeulike:3365807, abstract = {Many Gram-negative bacteria use a type I secretion system to translocate proteins, including pore-forming toxins, proteases, lipases and S-layer proteins, across both the inner and outer membranes into the extracellular surroundings. The Escherichia coli alpha-hemolysin (HlyA) secretion system is the prototypical and best characterized type I secretion system. The structure and function of the components of the HlyA secretion apparatus, HlyB, HlyD and TolC, have been studied in great detail. The functional characteristics of this secretion system enable it to be used in a variety of different applications, including the presentation of heterologous antigens in live-attenuated bacterial vaccines. Such vaccines can be an effective delivery system for heterologous antigens, and the use of a type I secretion system allows the antigens to be actively exported from the cytoplasm of the bacterial carrier rather than only becoming accessible to the host immune system after bacterial disintegration.}, author = {Gentschev, I. and Dietrich, G. and Goebel, W.}, citeulike-article-id = {3365807}, citeulike-linkout-0 = {http://view.ncbi.nlm.nih.gov/pubmed/11755084}, citeulike-linkout-1 = {http://www.hubmed.org/display.cgi?uids=11755084}, issn = {0966-842X}, journal = {Trends in microbiology}, keywords = {hemolysin, hlyd, tolc, toxin, type-i, vaccine}, month = {January}, number = {1}, pages = {39--45}, posted-at = {2008-10-02 11:40:45}, priority = {2}, title = {The E. coli alpha-hemolysin secretion system and its use in vaccine development.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/11755084}, volume = {10}, year = {2002} }

TY - JOUR ID - citeulike:3365807 L3 - citeulike-article-id:3365807 N2 - Many Gram-negative bacteria use a type I secretion system to translocate proteins, including pore-forming toxins, proteases, lipases and S-layer proteins, across both the inner and outer membranes into the extracellular surroundings. The Escherichia coli alpha-hemolysin (HlyA) secretion system is the prototypical and best characterized type I secretion system. The structure and function of the components of the HlyA secretion apparatus, HlyB, HlyD and TolC, have been studied in great detail. The functional characteristics of this secretion system enable it to be used in a variety of different applications, including the presentation of heterologous antigens in live-attenuated bacterial vaccines. Such vaccines can be an effective delivery system for heterologous antigens, and the use of a type I secretion system allows the antigens to be actively exported from the cytoplasm of the bacterial carrier rather than only becoming accessible to the host immune system after bacterial disintegration. IS - 1 JF - Trends in microbiology SN - 0966-842X EP - 45 TI - The E. coli alpha-hemolysin secretion system and its use in vaccine development. VL - 10 SP - 39 KW - hemolysin KW - hlyd KW - tolc KW - toxin KW - type-i KW - vaccine AU - Gentschev, I AU - Dietrich, G AU - Goebel, W PY - 2002/01// UR - http://view.ncbi.nlm.nih.gov/pubmed/11755084 ER -