Oral immunization of mice with recombinant Lactococcus lactis expressing porcine transmissible gastroenteritis virus spike glycoprotein. Export

@article{citeulike:5406279, abstract = {Lactococcus lactis NZ9000 was selected as an antigen delivery vehicle for mucosal immunization against porcine transmissible gastroenteritis virus (TGEV) infection. An approximately 70 kDa fragment of the N-terminal globular domain of the spike (S) protein (S(N) protein) from the coronavirus TGEV was used as the transmissible gastroenteritis virus antigen model. Recombinant L. lactis, expressing the S(N) protein, was constructed with the pNZ8112 plasmid. Expression and localization of the transcribed S(N) protein from the recombinant L(NZ)9000-rTGEV-S(N) were detected via SDS-PAGE, Western blot, and immunofluorescence. BALB/c mice, orally immunized with L(NZ)9000-rTGEV-S(N), produced local mucosal immune responses against TGEV. The induced antibodies demonstrated neutralizing effects on TGEV infection. These data indicated that the recombinant L. lactis could be a valuable tool in the development of future vaccines against TGEV.}, author = {Tang, Lijie and Li, Yijing}, citeulike-article-id = {5406279}, citeulike-linkout-0 = {http://dx.doi.org/10.1007/s11262-009-0390-x}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19629668}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19629668}, citeulike-linkout-3 = {http://www.springerlink.com/content/t190qmv63578x73t}, day = {24}, doi = {10.1007/s11262-009-0390-x}, journal = {Virus genes}, keywords = {expression, lactis, vaccine}, month = {July}, posted-at = {2009-11-06 13:05:57}, priority = {2}, title = {Oral immunization of mice with recombinant Lactococcus lactis expressing porcine transmissible gastroenteritis virus spike glycoprotein.}, url = {http://dx.doi.org/10.1007/s11262-009-0390-x}, year = {2009} }

TY - JOUR ID - citeulike:5406279 L3 - citeulike-article-id:5406279 N2 - Lactococcus lactis NZ9000 was selected as an antigen delivery vehicle for mucosal immunization against porcine transmissible gastroenteritis virus (TGEV) infection. An approximately 70 kDa fragment of the N-terminal globular domain of the spike (S) protein (S(N) protein) from the coronavirus TGEV was used as the transmissible gastroenteritis virus antigen model. Recombinant L. lactis, expressing the S(N) protein, was constructed with the pNZ8112 plasmid. Expression and localization of the transcribed S(N) protein from the recombinant L(NZ)9000-rTGEV-S(N) were detected via SDS-PAGE, Western blot, and immunofluorescence. BALB/c mice, orally immunized with L(NZ)9000-rTGEV-S(N), produced local mucosal immune responses against TGEV. The induced antibodies demonstrated neutralizing effects on TGEV infection. These data indicated that the recombinant L. lactis could be a valuable tool in the development of future vaccines against TGEV. JF - Virus genes TI - Oral immunization of mice with recombinant Lactococcus lactis expressing porcine transmissible gastroenteritis virus spike glycoprotein. KW - expression KW - lactis KW - vaccine AU - Tang, Lijie AU - Li, Yijing PY - 2009/07/24/ UR - http://dx.doi.org/10.1007/s11262-009-0390-x ER -