Identification of Birds through DNA Barcodes Export

@article{citeulike:139953, abstract = {Short DNA sequences from a standardized region of the genome provide a DNA barcode for identifying species. Compiling a public library of DNA barcodes linked to named specimens could provide a new master key for identifying species, one whose power will rise with increased taxon coverage and with faster, cheaper sequencing. Recent work suggests that sequence diversity in a 648-bp region of the mitochondrial gene, cytochrome c oxidase I (COI), might serve as a DNA barcode for the identification of animal species. This study tested the effectiveness of a COI barcode in discriminating bird species, one of the largest and best-studied vertebrate groups. We determined COI barcodes for 260 species of North American birds and found that distinguishing species was generally straightforward. All species had a different COI barcode(s), and the differences between closely related species were, on average, 18 times higher than the differences within species. Our results identified four probable new species of North American birds, suggesting that a global survey will lead to the recognition of many additional bird species. The finding of large COI sequence differences between, as compared to small differences within, species confirms the effectiveness of COI barcodes for the identification of bird species. This result plus those from other groups of animals imply that a standard screening threshold of sequence difference (10\~{A}— average intraspecific difference) could speed the discovery of new animal species. The growing evidence for the effectiveness of DNA barcodes as a basis for species identification supports an international exercise that has recently begun to assemble a comprehensive library of COI sequences linked to named specimens.}, address = {Department of Zoology, University of Guelph, Guelph, Ontario, Canada. phebert@uoguelph.ca.}, author = {Hebert, Paul D. N. and Stoeckle, Mark Y. and Zemlak, Tyler S. and Francis, Charles M.}, citeulike-article-id = {139953}, citeulike-linkout-0 = {http://dx.doi.org/10.1371/journal.pbio.0020312}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/15455034}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=15455034}, day = {28}, doi = {10.1371/journal.pbio.0020312}, issn = {1545-7885}, journal = {PLoS Biol}, keywords = {c, cytochrome, dna, variation}, month = {September}, number = {10}, pages = {e312+}, posted-at = {2005-03-25 16:08:30}, priority = {2}, publisher = {Public Library of Science}, title = {Identification of Birds through DNA Barcodes}, url = {http://dx.doi.org/10.1371/journal.pbio.0020312}, volume = {2}, year = {2004} }

TY - JOUR ID - citeulike:139953 L3 - citeulike-article-id:139953 N2 - Short DNA sequences from a standardized region of the genome provide a DNA barcode for identifying species. Compiling a public library of DNA barcodes linked to named specimens could provide a new master key for identifying species, one whose power will rise with increased taxon coverage and with faster, cheaper sequencing. Recent work suggests that sequence diversity in a 648-bp region of the mitochondrial gene, cytochrome c oxidase I (COI), might serve as a DNA barcode for the identification of animal species. This study tested the effectiveness of a COI barcode in discriminating bird species, one of the largest and best-studied vertebrate groups. We determined COI barcodes for 260 species of North American birds and found that distinguishing species was generally straightforward. All species had a different COI barcode(s), and the differences between closely related species were, on average, 18 times higher than the differences within species. Our results identified four probable new species of North American birds, suggesting that a global survey will lead to the recognition of many additional bird species. The finding of large COI sequence differences between, as compared to small differences within, species confirms the effectiveness of COI barcodes for the identification of bird species. This result plus those from other groups of animals imply that a standard screening threshold of sequence difference (10× average intraspecific difference) could speed the discovery of new animal species. The growing evidence for the effectiveness of DNA barcodes as a basis for species identification supports an international exercise that has recently begun to assemble a comprehensive library of COI sequences linked to named specimens. IS - 10 JF - PLoS Biol SN - 1545-7885 AD - Department of Zoology, University of Guelph, Guelph, Ontario, Canada. phebert@uoguelph.ca. TI - Identification of Birds through DNA Barcodes PB - Public Library of Science VL - 2 SP - e312 KW - c KW - cytochrome KW - dna KW - variation AU - Hebert, Paul AU - Stoeckle, Mark AU - Zemlak, Tyler AU - Francis, Charles PY - 2004/09/28/ UR - http://dx.doi.org/10.1371/journal.pbio.0020312 ER -