SAP18 promotes Krüppel-dependent transcriptional repression by enhancer-specific histone deacetylation. Export

@article{citeulike:4294340, abstract = {Body pattern formation during early embryogenesis of Drosophila melanogaster relies on a zygotic cascade of spatially restricted transcription factor activities. The gap gene Kr\"{u}ppel ranks at the top level of this cascade. It encodes a C2H2 zinc finger protein that interacts directly with cis-acting stripe enhancer elements of pair rule genes, such as even skipped and hairy, at the next level of the gene hierarchy. Kr\"{u}ppel mediates their transcriptional repression by direct association with the corepressor Drosophila C terminus-binding protein (dCtBP). However, for some Kr\"{u}ppel target genes, deletion of the dCtBP-binding sites does not abolish repression, implying a dCtBP-independent mode of repression. We identified Kr\"{u}ppel-binding proteins by mass spectrometry and found that SAP18 can both associate with Kr\"{u}ppel and support Kr\"{u}ppel-dependent repression. Genetic interaction studies combined with pharmacological and biochemical approaches suggest a site-specific mechanism of Kr\"{u}ppel-dependent gene silencing. The results suggest that Kr\"{u}ppel tethers the SAP18 bound histone deacetylase complex 1 at distinct enhancer elements, which causes repression via histone H3 deacetylation.}, author = {Matyash, A. and Singh, N. and Hanes, S. D. and Urlaub, H. and J\"{a}ckle, H.}, citeulike-article-id = {4294340}, citeulike-linkout-0 = {http://dx.doi.org/10.1074/jbc.M806163200}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/19049982}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=19049982}, day = {30}, doi = {10.1074/jbc.M806163200}, issn = {0021-9258}, journal = {The Journal of biological chemistry}, keywords = {chromatin, histones}, month = {January}, number = {5}, pages = {3012--3020}, posted-at = {2009-04-09 12:56:06}, priority = {2}, title = {SAP18 promotes Kr\"{u}ppel-dependent transcriptional repression by enhancer-specific histone deacetylation.}, url = {http://dx.doi.org/10.1074/jbc.M806163200}, volume = {284}, year = {2009} }

TY - JOUR ID - citeulike:4294340 L3 - citeulike-article-id:4294340 N2 - Body pattern formation during early embryogenesis of Drosophila melanogaster relies on a zygotic cascade of spatially restricted transcription factor activities. The gap gene Krüppel ranks at the top level of this cascade. It encodes a C2H2 zinc finger protein that interacts directly with cis-acting stripe enhancer elements of pair rule genes, such as even skipped and hairy, at the next level of the gene hierarchy. Krüppel mediates their transcriptional repression by direct association with the corepressor Drosophila C terminus-binding protein (dCtBP). However, for some Krüppel target genes, deletion of the dCtBP-binding sites does not abolish repression, implying a dCtBP-independent mode of repression. We identified Krüppel-binding proteins by mass spectrometry and found that SAP18 can both associate with Krüppel and support Krüppel-dependent repression. Genetic interaction studies combined with pharmacological and biochemical approaches suggest a site-specific mechanism of Krüppel-dependent gene silencing. The results suggest that Krüppel tethers the SAP18 bound histone deacetylase complex 1 at distinct enhancer elements, which causes repression via histone H3 deacetylation. IS - 5 JF - The Journal of biological chemistry SN - 0021-9258 EP - 3020 TI - SAP18 promotes Krüppel-dependent transcriptional repression by enhancer-specific histone deacetylation. VL - 284 SP - 3012 KW - chromatin KW - histones AU - Matyash, A AU - Singh, N AU - Hanes, SD AU - Urlaub, H AU - Jäckle, H PY - 2009/01/30/ UR - http://dx.doi.org/10.1074/jbc.M806163200 ER -