Identification of larval sea basses (Centropristis spp.) using ribosomal DNA-specific molecular assays Export

@article{citeulike:2883528, abstract = {The identification of sea bass (Centropristis) larvae to species is difficult because of similar morphological characters, spawning times, and overlapping species ranges. Black sea bass (Centropristis striata) is an important fishery species and is currently considered to be overfished south of Cape Hatteras, North Carolina. We describe methods for identifying three species of sea bass larvae using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) assays based on species-specific amplification of rDNA internal transcribed spacer reg ions. The assays were tested against DNA of ten other cooccurring reef fish species to ensure the assay's specificity. Centropristis larvae were collected on three cruises during cross-shelf transects and were used to validate the assays. Seventysix Centropristis larvae were assayed and 69 (91\%) were identified successfully. DNA was not amplified from 5\% of the larvae and identification was inconclusive for 3\% of the larvae. These assays can be used to identify sea bass eggs and larvae and will help to assess spawning locations, spawning times, and larval dispersal.}, author = {Vandersea, Mark W. and Litaker, Wayne R. and Marancik, Katrin E. and Hare, Jonathan A. and Walsh, Harvey J. and Lem, Siya and West, Melissa A. and Wyanski, David M. and Laban, Elisabeth H. and Tester, Patricia A.}, citeulike-article-id = {2883528}, journal = {Fishery Bulletin}, number = {2}, pages = {183--193}, posted-at = {2008-06-11 19:20:00}, priority = {2}, title = {Identification of larval sea basses (Centropristis spp.) using ribosomal DNA-specific molecular assays}, volume = {106}, year = {2008} }

TY - JOUR ID - citeulike:2883528 L3 - citeulike-article-id:2883528 N2 - The identification of sea bass (Centropristis) larvae to species is difficult because of similar morphological characters, spawning times, and overlapping species ranges. Black sea bass (Centropristis striata) is an important fishery species and is currently considered to be overfished south of Cape Hatteras, North Carolina. We describe methods for identifying three species of sea bass larvae using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) assays based on species-specific amplification of rDNA internal transcribed spacer reg ions. The assays were tested against DNA of ten other cooccurring reef fish species to ensure the assay’s specificity. Centropristis larvae were collected on three cruises during cross-shelf transects and were used to validate the assays. Seventysix Centropristis larvae were assayed and 69 (91%) were identified successfully. DNA was not amplified from 5% of the larvae and identification was inconclusive for 3% of the larvae. These assays can be used to identify sea bass eggs and larvae and will help to assess spawning locations, spawning times, and larval dispersal. IS - 2 JF - Fishery Bulletin EP - 193 TI - Identification of larval sea basses (Centropristis spp.) using ribosomal DNA-specific molecular assays VL - 106 SP - 183 AU - Vandersea, Mark AU - Litaker, Wayne AU - Marancik, Katrin AU - Hare, Jonathan AU - Walsh, Harvey AU - Lem, Siya AU - West, Melissa AU - Wyanski, David AU - Laban, Elisabeth AU - Tester, Patricia PY - 2008/// ER -