Bipolar, anastral spindle development in artificially activated sea urchin eggs Export

@article{citeulike:2883534, abstract = {The mitotic apparatus of the early sea urchin embryo is the archetype example of a centrosome-dominated, large aster spindle organized by means of the centriole of the fertilizing sperm. In this study, we tested the hypothesis that artificially activated sea urchin eggs possess the capacity to assemble the anastral, bipolar spindles present in many acentrosomal systems. Control fertilized Lytechinus pictus embryos and ammonia-activated eggs were immunolabeled for tubulin, centrosomal material, the spindle pole structuring protein NuMA and the mitotic kinesins MKLP1/Kinesin-6, Eg5/Kinesin-5, and KinI/Kinesin-13. Confocal imaging showed that a subset of ammonia-activated eggs contained bipolar mini-spindles that were anastral; displayed metaphase and anaphase-like stages; labeled for centrosomal material, NuMA, and the three mitotic kinesins; and were observed in living eggs using polarization optics. These results suggest that spindle structural and motor proteins have the ability to organize bipolar, anastral spindles in sea urchin eggs activated in the absence of the paternal centriole.}, author = {Henson, John H. and Fried, Christopher A. and Mcclellan, Mary K. and Ader, Jason and Davis, Jessica E. and Oldenbourg, Rudolf and Simerly, Calvin R.}, citeulike-article-id = {2883534}, journal = {Developmental Dynamics}, number = {5}, pages = {1348--1358}, posted-at = {2008-06-11 19:27:57}, priority = {2}, title = {Bipolar, anastral spindle development in artificially activated sea urchin eggs}, volume = {237}, year = {2008} }

TY - JOUR ID - citeulike:2883534 L3 - citeulike-article-id:2883534 N2 - The mitotic apparatus of the early sea urchin embryo is the archetype example of a centrosome-dominated, large aster spindle organized by means of the centriole of the fertilizing sperm. In this study, we tested the hypothesis that artificially activated sea urchin eggs possess the capacity to assemble the anastral, bipolar spindles present in many acentrosomal systems. Control fertilized Lytechinus pictus embryos and ammonia-activated eggs were immunolabeled for tubulin, centrosomal material, the spindle pole structuring protein NuMA and the mitotic kinesins MKLP1/Kinesin-6, Eg5/Kinesin-5, and KinI/Kinesin-13. Confocal imaging showed that a subset of ammonia-activated eggs contained bipolar mini-spindles that were anastral; displayed metaphase and anaphase-like stages; labeled for centrosomal material, NuMA, and the three mitotic kinesins; and were observed in living eggs using polarization optics. These results suggest that spindle structural and motor proteins have the ability to organize bipolar, anastral spindles in sea urchin eggs activated in the absence of the paternal centriole. IS - 5 JF - Developmental Dynamics EP - 1358 TI - Bipolar, anastral spindle development in artificially activated sea urchin eggs VL - 237 SP - 1348 AU - Henson, John AU - Fried, Christopher AU - Mcclellan, Mary AU - Ader, Jason AU - Davis, Jessica AU - Oldenbourg, Rudolf AU - Simerly, Calvin PY - 2008/// ER -