Shear stress inhibits while disuse promotes osteocyte apoptosis Export

@article{citeulike:3479548, abstract = {Cell apoptosis operates as an organizing mechanism in biology in addition to removing effete cells. We have recently proposed that during bone remodeling, osteocyte apoptosis steers osteonal alignment in relation to mechanical loading of the whole bone [J. Biomech. 36 (2003) 1453]. Here we present evidence that osteocyte apoptosis in cell culture is modulated by shear stress. Under static culture conditions, serum starved osteocytes exposed phosphatidylserine (PS) on their cell membrane 6\&\#xd7; more often than periosteal fibroblasts and 3\&\#xd7; more often than osteoblasts. Treatment with shear stress reduced the number of osteocytes that exposed PS by 90\%, but did not affect the other cell types. Fluid shear stress of increasing magnitude, dose-dependently stimulated Bcl-2 mRNA expression in human bone cells, while shear stress did not change Bax expression. These data suggest that disuse promotes osteocyte apoptosis, while mechanical stimulation by fluid shear stress promotes osteocyte survival, by modulating the Bcl-2/Bax expression ratio.}, author = {Bakker, A.}, citeulike-article-id = {3479548}, citeulike-linkout-0 = {http://dx.doi.org/10.1016/j.bbrc.2004.06.056}, citeulike-linkout-1 = {http://linkinghub.elsevier.com/retrieve/pii/S0006-291X(04)01341-5}, doi = {10.1016/j.bbrc.2004.06.056}, issn = {0006291X}, journal = {Biochemical and Biophysical Research Communications}, keywords = {apoptosis, osteocytes, shear}, month = {August}, number = {4}, pages = {1163--1168}, posted-at = {2008-11-04 17:26:18}, priority = {2}, title = {Shear stress inhibits while disuse promotes osteocyte apoptosis}, url = {http://dx.doi.org/10.1016/j.bbrc.2004.06.056}, volume = {320}, year = {2004} }

TY - JOUR ID - citeulike:3479548 L3 - citeulike-article-id:3479548 N2 - Cell apoptosis operates as an organizing mechanism in biology in addition to removing effete cells. We have recently proposed that during bone remodeling, osteocyte apoptosis steers osteonal alignment in relation to mechanical loading of the whole bone [J. Biomech. 36 (2003) 1453]. Here we present evidence that osteocyte apoptosis in cell culture is modulated by shear stress. Under static culture conditions, serum starved osteocytes exposed phosphatidylserine (PS) on their cell membrane 6× more often than periosteal fibroblasts and 3× more often than osteoblasts. Treatment with shear stress reduced the number of osteocytes that exposed PS by 90%, but did not affect the other cell types. Fluid shear stress of increasing magnitude, dose-dependently stimulated Bcl-2 mRNA expression in human bone cells, while shear stress did not change Bax expression. These data suggest that disuse promotes osteocyte apoptosis, while mechanical stimulation by fluid shear stress promotes osteocyte survival, by modulating the Bcl-2/Bax expression ratio. IS - 4 JF - Biochemical and Biophysical Research Communications SN - 0006291X EP - 1168 TI - Shear stress inhibits while disuse promotes osteocyte apoptosis VL - 320 SP - 1163 KW - apoptosis KW - osteocytes KW - shear AU - Bakker, A PY - 2004/08// UR - http://dx.doi.org/10.1016/j.bbrc.2004.06.056 ER -