Salmonella enterica serovar typhimurium exploits inflammation to compete with the intestinal microbiota. Export

@article{citeulike:2672233, abstract = {Most mucosal surfaces of the mammalian body are colonized by microbial communities ("microbiota"). A high density of commensal microbiota inhabits the intestine and shields from infection ("colonization resistance"). The virulence strategies allowing enteropathogenic bacteria to successfully compete with the microbiota and overcome colonization resistance are poorly understood. Here, we investigated manipulation of the intestinal microbiota by the enteropathogenic bacterium Salmonella enterica subspecies 1 serovar Typhimurium (S. Tm) in a mouse colitis model: we found that inflammatory host responses induced by S. Tm changed microbiota composition and suppressed its growth. In contrast to wild-type S. Tm, an avirulent invGsseD mutant failing to trigger colitis was outcompeted by the microbiota. This competitive defect was reverted if inflammation was provided concomitantly by mixed infection with wild-type S. Tm or in mice (IL10(-/-), VILLIN-HA(CL4-CD8)) with inflammatory bowel disease. Thus, inflammation is necessary and sufficient for overcoming colonization resistance. This reveals a new concept in infectious disease: in contrast to current thinking, inflammation is not always detrimental for the pathogen. Triggering the host's immune defence can shift the balance between the protective microbiota and the pathogen in favour of the pathogen.}, address = {Institute of Microbiology, Swiss Institute of Technology Zurich, Zurich, Switzerland.}, author = {Stecher, B. and Robbiani, R. and Walker, A. W. and Westendorf, A. M. and Barthel, M. and Kremer, M. and Chaffron, S. and Macpherson, A. J. and Buer, J. and Parkhill, J. and Dougan, G. and von Mering, C. and Hardt, W. D.}, citeulike-article-id = {2672233}, citeulike-linkout-0 = {http://dx.doi.org/10.1371/journal.pbio.0050244}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/17760501}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=17760501}, doi = {10.1371/journal.pbio.0050244}, issn = {1545-7885}, journal = {PLoS biology}, keywords = {methods, mice, salmonella}, month = {October}, number = {10}, pages = {2177--2189}, posted-at = {2008-04-15 09:15:36}, priority = {2}, title = {Salmonella enterica serovar typhimurium exploits inflammation to compete with the intestinal microbiota.}, url = {http://dx.doi.org/10.1371/journal.pbio.0050244}, volume = {5}, year = {2007} }

TY - JOUR ID - citeulike:2672233 L3 - citeulike-article-id:2672233 N2 - Most mucosal surfaces of the mammalian body are colonized by microbial communities ("microbiota"). A high density of commensal microbiota inhabits the intestine and shields from infection ("colonization resistance"). The virulence strategies allowing enteropathogenic bacteria to successfully compete with the microbiota and overcome colonization resistance are poorly understood. Here, we investigated manipulation of the intestinal microbiota by the enteropathogenic bacterium Salmonella enterica subspecies 1 serovar Typhimurium (S. Tm) in a mouse colitis model: we found that inflammatory host responses induced by S. Tm changed microbiota composition and suppressed its growth. In contrast to wild-type S. Tm, an avirulent invGsseD mutant failing to trigger colitis was outcompeted by the microbiota. This competitive defect was reverted if inflammation was provided concomitantly by mixed infection with wild-type S. Tm or in mice (IL10(-/-), VILLIN-HA(CL4-CD8)) with inflammatory bowel disease. Thus, inflammation is necessary and sufficient for overcoming colonization resistance. This reveals a new concept in infectious disease: in contrast to current thinking, inflammation is not always detrimental for the pathogen. Triggering the host's immune defence can shift the balance between the protective microbiota and the pathogen in favour of the pathogen. IS - 10 JF - PLoS biology SN - 1545-7885 AD - Institute of Microbiology, Swiss Institute of Technology Zurich, Zurich, Switzerland. EP - 2189 TI - Salmonella enterica serovar typhimurium exploits inflammation to compete with the intestinal microbiota. VL - 5 SP - 2177 KW - methods KW - mice KW - salmonella AU - Stecher, B AU - Robbiani, R AU - Walker, AW AU - Westendorf, AM AU - Barthel, M AU - Kremer, M AU - Chaffron, S AU - Macpherson, AJ AU - Buer, J AU - Parkhill, J AU - Dougan, G AU - von Mering, C AU - Hardt, WD PY - 2007/10// UR - http://dx.doi.org/10.1371/journal.pbio.0050244 ER -