4Pi-confocal microscopy provides three-dimensional images of the microtubule network with 100- to 150-nm resolution. Export

@article{citeulike:2624164, abstract = {We show the applicability of 4Pi-confocal microscopy to three-dimensional imaging of the microtubule network in a fixed mouse fibroblast cell. Comparison with two-photon confocal resolution reveals a fourfold better axial resolution in the 4Pi-confocal case. By combining 4Pi-confocal microscopy with Richardson-Lucy image restoration a further resolution increase is achieved. Featuring a three-dimensional resolution in the range 100-150 nm, the 4Pi-confocal (restored) images are intrinsically more detailed than their confocal counterparts. Our images constitute what to our knowledge are the best-resolved three-dimensional images of entangled cellular microtubules obtained with light to date.}, address = {High Resolution Optical Microscopy Group, Max-Planck-Institute for Biophysical Chemistry, G\"{o}ttingen, D-37070, Germany.}, author = {Nagorni, M. and Hell, S. W.}, citeulike-article-id = {2624164}, citeulike-linkout-0 = {http://dx.doi.org/10.1006/jsbi.1998.4037}, citeulike-linkout-1 = {http://view.ncbi.nlm.nih.gov/pubmed/9878578}, citeulike-linkout-2 = {http://www.hubmed.org/display.cgi?uids=9878578}, doi = {10.1006/jsbi.1998.4037}, issn = {1047-8477}, journal = {Journal of structural biology}, keywords = {4pi, confocal, fluorescence, quals, superresolution}, month = {November}, number = {3}, pages = {236--247}, posted-at = {2008-04-02 19:07:51}, priority = {2}, title = {4Pi-confocal microscopy provides three-dimensional images of the microtubule network with 100- to 150-nm resolution.}, url = {http://dx.doi.org/10.1006/jsbi.1998.4037}, volume = {123}, year = {1998} }

TY - JOUR ID - citeulike:2624164 L3 - citeulike-article-id:2624164 N2 - We show the applicability of 4Pi-confocal microscopy to three-dimensional imaging of the microtubule network in a fixed mouse fibroblast cell. Comparison with two-photon confocal resolution reveals a fourfold better axial resolution in the 4Pi-confocal case. By combining 4Pi-confocal microscopy with Richardson-Lucy image restoration a further resolution increase is achieved. Featuring a three-dimensional resolution in the range 100-150 nm, the 4Pi-confocal (restored) images are intrinsically more detailed than their confocal counterparts. Our images constitute what to our knowledge are the best-resolved three-dimensional images of entangled cellular microtubules obtained with light to date. IS - 3 JF - Journal of structural biology SN - 1047-8477 AD - High Resolution Optical Microscopy Group, Max-Planck-Institute for Biophysical Chemistry, Göttingen, D-37070, Germany. EP - 247 TI - 4Pi-confocal microscopy provides three-dimensional images of the microtubule network with 100- to 150-nm resolution. VL - 123 SP - 236 KW - 4pi KW - confocal KW - fluorescence KW - quals KW - superresolution AU - Nagorni, M AU - Hell, SW PY - 1998/11// UR - http://dx.doi.org/10.1006/jsbi.1998.4037 ER -