Fluorescence imaging with one nanometer accuracy: application to molecular motors. Export

@article{citeulike:2678491, abstract = {We introduce the technique of FIONA, fluorescence imaging with one nanometer accuracy. This is a fluorescence technique that is able to localize the position of a single dye within approximately 1 nm in the x-y plane. It is done simply by taking the point spread function of a single fluorophore excited with wide field illumination and locating the center of the fluorescent spot by a two-dimensional Gaussian fit. We motivate the development of FIONA by unraveling the walking mechanism of the molecular motors myosin V, myosin VI, and kinesin. We find that they all walk in a hand-over-hand fashion.}, address = {Center for Biophysics and Computational Biology and Department of Physics, University of Illinois, Urbana-Champaign, Illinois 61801, USA.}, author = {Yildiz, A. and Selvin, P. R.}, citeulike-article-id = {2678491}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/ar040136s}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/ar040136s}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/16028892}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=16028892}, doi = {10.1021/ar040136s}, issn = {0001-4842}, journal = {Accounts of chemical research}, keywords = {fiona, molecular-motors, myosin, quals, superresolution}, month = {July}, number = {7}, pages = {574--582}, posted-at = {2008-04-16 17:36:04}, priority = {0}, title = {Fluorescence imaging with one nanometer accuracy: application to molecular motors.}, url = {http://dx.doi.org/10.1021/ar040136s}, volume = {38}, year = {2005} }

TY - JOUR ID - citeulike:2678491 L3 - citeulike-article-id:2678491 N2 - We introduce the technique of FIONA, fluorescence imaging with one nanometer accuracy. This is a fluorescence technique that is able to localize the position of a single dye within approximately 1 nm in the x-y plane. It is done simply by taking the point spread function of a single fluorophore excited with wide field illumination and locating the center of the fluorescent spot by a two-dimensional Gaussian fit. We motivate the development of FIONA by unraveling the walking mechanism of the molecular motors myosin V, myosin VI, and kinesin. We find that they all walk in a hand-over-hand fashion. IS - 7 JF - Accounts of chemical research SN - 0001-4842 AD - Center for Biophysics and Computational Biology and Department of Physics, University of Illinois, Urbana-Champaign, Illinois 61801, USA. EP - 582 TI - Fluorescence imaging with one nanometer accuracy: application to molecular motors. VL - 38 SP - 574 KW - fiona KW - molecular-motors KW - myosin KW - quals KW - superresolution AU - Yildiz, A AU - Selvin, PR PY - 2005/07// UR - http://dx.doi.org/10.1021/ar040136s ER -