Lifeact: a versatile marker to visualize F-actin. Export

@article{citeulike:2910461, abstract = {Live imaging of the actin cytoskeleton is crucial for the study of many fundamental biological processes, but current approaches to visualize actin have several limitations. Here we describe Lifeact, a 17-amino-acid peptide, which stained filamentous actin (F-actin) structures in eukaryotic cells and tissues. Lifeact did not interfere with actin dynamics in vitro and in vivo and in its chemically modified peptide form allowed visualization of actin dynamics in nontransfectable cells.}, address = {[1] Max Planck Institute of Biochemistry, Independent Junior Research Group Cellular Dynamics and Cell Patterning, Am Klopferspitz 18, 82152 Martinsried, Germany. [2] These authors contributed equally to this work.}, author = {Riedl, Julia and Crevenna, Alvaro H H. and Kessenbrock, Kai and Yu, Jerry Haochen H. and Neukirchen, Dorothee and Bista, Michal and Bradke, Frank and Jenne, Dieter and Holak, Tad A A. and Werb, Zena and Sixt, Michael and Wedlich-Soldner, Roland}, citeulike-article-id = {2910461}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nmeth.1220}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nmeth.1220}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/18536722}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=18536722}, day = {8}, doi = {10.1038/nmeth.1220}, issn = {1548-7105}, journal = {Nature methods}, keywords = {actin, actin-label, f-actin, fluorescent-tag}, month = {June}, posted-at = {2008-07-17 23:56:40}, priority = {2}, title = {Lifeact: a versatile marker to visualize F-actin.}, url = {http://dx.doi.org/10.1038/nmeth.1220}, year = {2008} }

TY - JOUR ID - citeulike:2910461 L3 - citeulike-article-id:2910461 N2 - Live imaging of the actin cytoskeleton is crucial for the study of many fundamental biological processes, but current approaches to visualize actin have several limitations. Here we describe Lifeact, a 17-amino-acid peptide, which stained filamentous actin (F-actin) structures in eukaryotic cells and tissues. Lifeact did not interfere with actin dynamics in vitro and in vivo and in its chemically modified peptide form allowed visualization of actin dynamics in nontransfectable cells. JF - Nature methods SN - 1548-7105 AD - [1] Max Planck Institute of Biochemistry, Independent Junior Research Group Cellular Dynamics and Cell Patterning, Am Klopferspitz 18, 82152 Martinsried, Germany. [2] These authors contributed equally to this work. TI - Lifeact: a versatile marker to visualize F-actin. KW - actin KW - actin-label KW - f-actin KW - fluorescent-tag AU - Riedl, Julia AU - Crevenna, Alvaro H AU - Kessenbrock, Kai AU - Yu, Jerry Haochen AU - Neukirchen, Dorothee AU - Bista, Michal AU - Bradke, Frank AU - Jenne, Dieter AU - Holak, Tad A AU - Werb, Zena AU - Sixt, Michael AU - Wedlich-Soldner, Roland PY - 2008/06/08/ UR - http://dx.doi.org/10.1038/nmeth.1220 ER -