Fluorescent probes for super-resolution imaging in living cells Export

@article{citeulike:3646870, abstract = {In 1873, Ernst Abbe discovered that features closer than 200 nm cannot be resolved by lens-based light microscopy. In recent years, however, several new far-field super-resolution imaging techniques have broken this diffraction limit, producing, for example, video-rate movies of synaptic vesicles in living neurons with 62 nm spatial resolution. Current research is focused on further improving spatial resolution in an effort to reach the goal of video-rate imaging of live cells with molecular (1–5 nm) resolution. Here, we describe the contributions of fluorescent probes to far-field super-resolution imaging, focusing on fluorescent proteins and organic small-molecule fluorophores. We describe the features of existing super-resolution fluorophores and highlight areas of importance for future research and development.}, author = {Fernandez-Suarez, Marta and Ting, Alice Y.}, citeulike-article-id = {3646870}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nrm2531}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nrm2531}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19002208}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19002208}, day = {12}, doi = {10.1038/nrm2531}, issn = {1471-0072}, journal = {Nature Reviews Molecular Cell Biology}, keywords = {fluorophore, review, superresolution}, month = {November}, number = {12}, pages = {929--943}, posted-at = {2008-11-21 19:11:32}, priority = {2}, publisher = {Nature Publishing Group}, title = {Fluorescent probes for super-resolution imaging in living cells}, url = {http://dx.doi.org/10.1038/nrm2531}, volume = {9}, year = {2008} }

TY - JOUR ID - citeulike:3646870 L3 - citeulike-article-id:3646870 N2 - In 1873, Ernst Abbe discovered that features closer than 200 nm cannot be resolved by lens-based light microscopy. In recent years, however, several new far-field super-resolution imaging techniques have broken this diffraction limit, producing, for example, video-rate movies of synaptic vesicles in living neurons with 62 nm spatial resolution. Current research is focused on further improving spatial resolution in an effort to reach the goal of video-rate imaging of live cells with molecular (1–5 nm) resolution. Here, we describe the contributions of fluorescent probes to far-field super-resolution imaging, focusing on fluorescent proteins and organic small-molecule fluorophores. We describe the features of existing super-resolution fluorophores and highlight areas of importance for future research and development. IS - 12 JF - Nature Reviews Molecular Cell Biology SN - 1471-0072 EP - 943 TI - Fluorescent probes for super-resolution imaging in living cells PB - Nature Publishing Group VL - 9 SP - 929 KW - fluorophore KW - review KW - superresolution AU - Fernandez-Suarez, Marta AU - Ting, Alice PY - 2008/11/12/ UR - http://dx.doi.org/10.1038/nrm2531 ER -