Design of DNA-conjugated polypeptide-based capture probes for the anchoring of proteins to DNA matrices. Export

@article{citeulike:3889532, abstract = {A new method for protein surface functionalization was developed that utilizes DNA-conjugated artificial polypeptides to capture recombinant target proteins from the solution phase and direct their deposition onto DNA-functionalized matrices. Protein capture is accomplished through the coiled-coil association of an engineered pair of heterodimeric leucine zippers. Incorporating half of the zipper complex directly into the polypeptides and labeling these polymers with ssDNA enables the polypeptide conjugates to form intermediate linkages that connect the target proteins securely to DNA-functionalized supports. This synthetic route provides an important alternative to conventional DNA-conjugation techniques by allowing proteins to be outfitted site-specifically with ssDNA while minimizing the need for postexpression processing. We demonstrate these attributes by (i) using the capture probes to prepare protein microarrays, (ii) demonstrating control over enzyme activity via deposition of DNA, and, (iii) synthesizing finite-sized, multiprotein complexes that are templated on designed DNA scaffolds in near quantitative yield.}, author = {Schweller, R. M. and Constantinou, P. E. and Frankel, N. W. and Narayan, P. and Diehl, M. R.}, citeulike-article-id = {3889532}, citeulike-linkout-0 = {http://dx.doi.org/10.1021/bc8003606}, citeulike-linkout-1 = {http://pubs.acs.org/doi/abs/10.1021/bc8003606}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/19053307}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=19053307}, doi = {10.1021/bc8003606}, issn = {1520-4812}, journal = {Bioconjugate chemistry}, keywords = {dna, dna-scaffold, motor-array, multiple-motors, scaffold}, month = {December}, number = {12}, pages = {2304--2307}, posted-at = {2009-01-15 19:32:50}, priority = {2}, title = {Design of DNA-conjugated polypeptide-based capture probes for the anchoring of proteins to DNA matrices.}, url = {http://dx.doi.org/10.1021/bc8003606}, volume = {19}, year = {2008} }

TY - JOUR ID - citeulike:3889532 L3 - citeulike-article-id:3889532 N2 - A new method for protein surface functionalization was developed that utilizes DNA-conjugated artificial polypeptides to capture recombinant target proteins from the solution phase and direct their deposition onto DNA-functionalized matrices. Protein capture is accomplished through the coiled-coil association of an engineered pair of heterodimeric leucine zippers. Incorporating half of the zipper complex directly into the polypeptides and labeling these polymers with ssDNA enables the polypeptide conjugates to form intermediate linkages that connect the target proteins securely to DNA-functionalized supports. This synthetic route provides an important alternative to conventional DNA-conjugation techniques by allowing proteins to be outfitted site-specifically with ssDNA while minimizing the need for postexpression processing. We demonstrate these attributes by (i) using the capture probes to prepare protein microarrays, (ii) demonstrating control over enzyme activity via deposition of DNA, and, (iii) synthesizing finite-sized, multiprotein complexes that are templated on designed DNA scaffolds in near quantitative yield. IS - 12 JF - Bioconjugate chemistry SN - 1520-4812 EP - 2307 TI - Design of DNA-conjugated polypeptide-based capture probes for the anchoring of proteins to DNA matrices. VL - 19 SP - 2304 KW - dna KW - dna-scaffold KW - motor-array KW - multiple-motors KW - scaffold AU - Schweller, RM AU - Constantinou, PE AU - Frankel, NW AU - Narayan, P AU - Diehl, MR PY - 2008/12// UR - http://dx.doi.org/10.1021/bc8003606 ER -